Clampex 10 expands the range and quality of your data acquisition experiments. These improvements should prove useful for a wide variety of applications, allowing more flexibility in your experimental protocols.

New Features

• Membrane Test calculates Ra and Cm per sweep during a recording

• Membrane and Seal Tests combined into a single resizable window

• Support for four stimulus waveforms (with the Digidata 1440A)

• Control of eight digital outputs per epoch during a sweep

• Control of split-clock sampling per epoch during a sweep

• Leak subtraction automatically saves both raw and corrected traces

• All protocol durations are entered in time units

• Support of the new Digidata 1440A digitizer

• Support of the new Axoclamp 900A amplifier

• New ABF 2.0 file format

Existing functionality includes the ability to automate the execution of protocols, a variety of recording modes from continuous to waveform stimulation, online filtering and leak subtraction, and online statistics/graphs.

AxoScope 10 provides a convenient way to produce background recordings with the included MiniDigi 2-channel digitizer*. You can monitor cells during intra-sweep periods, or create an overview of the entire day's activities including voice tags

Clampfit 10 is a powerful solution for analyzing, graphing and layout of all of your Clampex and AxoScope data. Clampfit includes an extensive array of filtering and fitting routines. Functionality includes I-V graphs, power spectrums, and special "linked data views" for threshold (AP), template (minis) and single-channel modes of event detection and analysis.

Applications

pCLAMP 10 is suitable for a wide variety of applications, ranging from electrophysiology to general physiology, from individual cells to networked cells, and from low-noise patch clamping to high-speed sodium channels.

Uses:

• Action Potentials (APs)

• Current-clamp

• Electromyography (EMG)

• Electrooculography (EOG)

• Excitatory Post-Synaptic Currents (EPSCs)

• Excitatory Post-Synaptic Potentials (EPSPs)

• Inhibitory Post-Synaptic Currents (IPSCs)

• Inhibitory Post-Synaptic Potentials (IPSPs)

• Long-Term Depression (LTD)

• Long-Term Potentiation (LTP)

• Miniature excitatory potentials (Minis)

• Ratio dyes (with PMTs)

• Single-channels

• Slices

• Spike trains

• Synaptic networks

• Voltage-clamp

• Whole-cell

* pCLAMP 9 included a MiniDigi digitizer, so upgrades from pCLAMP 9 to pCLAMP 10 do not include an additional MiniDigi digitizer.