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Perfusion Chamber I
This
perfusion chamber is designed for oocyte two-electrode voltage
clamp and patch clamp. Oocyte is confined in a cone shaped
well to a pixed position. Since the volume of perfusion chamber's
oocyte well is only 4 microliter, the solution change is
almost instantaneous. The solution outlet is in an isolated
chamber; the cell chamber does not experience the water surface
vibration due to the suction. The seal is thus unlikely to
be broken by the solution change. The solution level is determined
by a "water dam," which ensures a constant solution
level.
Perfusion Chamber II
The perfusion chamber is designed for oocyte excised patch and tissue culture
cells. Two 3-barrel perfusion pipettes are mounted on the perfusion chamber in
45 degree angle in the path of electrodes movement. Software can control electrodes
to stop at the center of each barrel for solution change. The time it takes to
move between the center of the barrels (700 microns) is 140 ms. For faster solution
change, people can pull 3-barrel glasses to smaller size. A small cone shaped
well confines the oocyte to a fixed position. Two confined locations are designed
for cell suspensions. Cultured cells on cover slip can be put on the flat bottom
of the dish. A suction tube is isolated from the cells so that solution vibration
from suction will not affect the oocyte.

Amplifier Headstage Adaptor
Adaptors for amplifiers made by Molecular Devices, Warner Instruments,
HEKA and other companies. Contact to confirm your amplfier
is supported.
Perfusion Chamber Support Complex
The perfusion chamber support complex includes a perfusion
chamber I, a stand, a perfusion chamber base plate, and a clamp
to fix the perfusion chamber onto the base plate.
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Related
products:
ezPATCH 100A
PatchMAX 100A
ChannelMAX 100A Mini
ChannelMAX
100A Twin |
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