ezPATCH 100A
The ezPatch 100A is a smart manipulator. It
has all the functions a manual manipulator has. In addition,
it comes with a computer controlled feedback system so that
it can stop precisely after electrode touches cell membrane.
The system is ideal for patch clamp.
In patch clamp experiments,
all users need to do is aim the electrode at a selected cell.
A click of a button and the electrode will stop at the surface
of the cell membrane in specified speed and pressure (measured
by resistance increase). Patch clamp experiments will be
consistent and precise once you find the best seal parameters.
The seal parameters can be saved for different cell types
and shared among lab members.
The control software has many
functions that make patch clamp experiments easy and precise.
You can excise patches at the predefined speed. With the
manual control functions in the software, you may move the
electrode forward or backward as small as 0.1 µm in defined
speed. The software can also control multiple manipulators.
Therefore users can make seals on the same cell or a few different
cells simultaneously.
If drift from pipette holder/o-ring
is a concern, an optional pipette holder clamp can be purchased.
It clamps the glass pipette so that the drift caused by o-ring
is minimal.
There is no additional electrical noise from
the manipulator since no current is going through the motor
when it stops. The friction can ensure that the motor does
not loose even a micro step gear (50 nm).
The BNC interface
box comes with 10 Analog INs and 2 Analog OUTs. A trigger
is provided for sync in case needed. For the best grounding
result, software can control the BNC interface box to be
either in float or grounded condition.
The ezPATCH 100A comes
with National Instruments' PCI 6221 DAQ board which is supported
by many data acquisition software such as jCLAMP. If you
plan to choose the software you will not need to purchase
extra data acquisition board.
Key Features
• Includes a computer
controlled manipulator, a data acquisition board, a BNC interface
box, and a control software.
• Resolution of the computer controlled
manipulator is 50 nm.
• Manipulator movement stops when patch
pipette touches cell membrane.
• No electrical noise during
recording.
• Ideal for patch clamp.
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