Polymer Microspheres (microparticles)
PolyAn‘s transparent or fluorescence encoded polymer microparticles are used for multiplex molecular diagnostics and LifeScience research. The polymer microspheres are comprised of thermally stable PMMA (Polymethylmethacrylate) which allows operating temperatures of up to 100°C. They can be functionalized with a reactive 3D-matrix for coupling of biomolecules.
Products
Transparent, functionalized polymer microparticles
PolyAn offers functionalized, transparent polymer beads with very narrow particle size distribution for particle analysis using flow cytometry or other screening applications:
| Product ID |
Mean Diameter [µm] |
Coefficient of Variation [%] |
Functional Group |
Solids content (w/v) [%] |
| 105 01 005 |
5 |
2 - 5 |
3D-Carboxy |
5 |
| 105 01 012 |
12 |
2 - 5 |
3D-Carboxy |
5 |
|
Other particle sizes and functional groups with different loading capacities are available upon request. Please do not hesitate to contact us.
Fluorescence dye labeled, functionalized polymer microparticles
PolyAn has developed polymer beads which are encoded using the combination of two different fluorescence dyes. They can be used in flow cytometry and / or image based systems like the Bioresponse / Aklydes plattform as well as in other screening applications.
| Product ID |
Color Labelling
| Dye 1 (Excitation / Emission) |
Dye 2 (Excitation / Emission) |
|
Functional Group |
Mean
Diameter
[µm] |
Coefficient of Variation [%] |
Solids content (w/v) [%] |
| 105 02 005 |
420-480nm / 485-540nm |
515-540nm / 535-570nm |
3D-Carboxy |
5 |
2 - 5 |
0,5 |
| 105 02 012 |
420-480nm / 485-540nm |
515-540nm / 535-570nm |
3D-Carboxy |
12 |
2 - 5 |
1,0 |
|
Other functional groups and microparticle sizes are available upon request.
PMMA microparticles: Size distribution
Standard product size range: 5-12 µm
Size uniformity specification: CV < 5%
 |
| Size and size distribution of 5 µm microparticles (CV < 2.5 %) |
Size and size distribution of 12 µm microparticles (CV < 3.8 %) |
|
Technology
PMMA (poly methyl methacrylate) core with a covalently bound reactive 3-dimensional surface.
|
| SEM image of PolyAn’s microparticles |
Schematic model of the 3D reactive matrix on the core polymer microparticle |
|
Density: 1.18 g/cm3
Glass transition temperature: Tg 110°C
PMMA microparticles: fluorescence encoding
PolyAn‘s PMMA microparticles are carboxy-functionalized using PolyAn‘s Molecular Surface Engineering Technology (MSE). They can be color-coded with up to two fluorescent dyes using PolyAn‘s proprietary process which incorporates the dye directly into the polymer during the particle production. By combining the two-color labelling with the bead size more than 100 populations are possible which can for example be used for multi parameter diagnostics (multiplex diagnostics).
| |
Emmission |
Emmission |
| Dye 1 |
420 - 480 nm |
485 - 540 nm |
| Dye 2 |
515 - 540 nm |
535 – 570 nm |
|
A combination of Pop ID´s with the microbead size
allows an even higher number of populations |
PolyAn’s beads are characterized by a High Signal-to-Noise Ratio that is achieved through a combination of high loading and low unspecific binding:
| Dynamic range up to 400 |
PolyAn´s MSE-functionalization ensures a high binding capacity and easy accessibility to the functional groups. The combination of reactive functional groups with PolyAn´s antifouling matrix allows an excellent signal-to-noise ratio. |
| Good optical properties |
The PolyAn substrate is characterized by a very low background fluorescence and good optical properties. |
| Stable at high temperatures |
Through the use of MSE, leakage of the dyes at high temperatures is avoided. PolyAn polymer microspheres are thermally stable up to 100°C. |
|
