µ-Slide Chemotaxis
µ-Slide for stable gradients, 3 chambers on
one slide for chemotaxis and migration assays
• long term chemotaxis experiments with adherent
cells
•
ready to use system, no assembling
•
linear gradients, stable for over 48 hours
•
3 chambers on one slide for parallel working
• adapters for different pipettes
•
made for high-end video microscopy
Basic Principle
• Two large reservoirs
of 40µl
are connected by a narrow observation area
• The cells in the
observation area become super-imposed by a linear and time-stable
gradient
Example Experiment
Catalog
Numbers: |
|
ibiTreat,
tissue culture treated, sterile |
|
Coated
(Collagen IV), sterile |
80302 |
Hydrophobic,
uncoated, sterile |
80301 |
|
Technical
Details: |
|
Chemotaxis
chambers on slide |
|
Volume
of the channel |
80 µl |
| Observation
area |
2x1
mm2 |
| Total
height with plugs |
12
mm |
| Volume
chemoattractant |
18 µl |
| Bottom
matches coverslip |
No.
1.5 |
|
|
