IonFlux HT & 16 - High throughput
automated patch clamping system
IonFlux provides an automated, high throughput solution for
ion channel drug discovery and research. The system utilizes
Fluxion's Well Plate Microfluidics™ (WPM) to conduct
high content patch clamping analysis in a convenient and flexible
well plate format.
Ion channels represent an important yet under-represented
drug target class due to the lack of available high-throughput
screening approaches. Manual patch-clamping, the “gold standard” for
performing cell electrophysiology, is a labor-intensive
technique that is too slow to be used for drug screening, while first-generation
automated electrophysiology systems are expensive and performance-challenged.
Fluxion has addressed these limitations with the IonFlux Automated Electrophysiology
System. The IonFlux system eliminates the need for intermediate pipetting steps
during the recording protocol and allows the system to be configured like a plate
reader, simplifying workflow and increasing throughput. The IonFlux system delivers
these advantages:
• The highest level of throughput (to
10,000 data points per day)
• Suitable for use with all ion channels including ligand-gated (compound exchange <50ms)
• The lowest cost per data point (as low as $0.30 per data point)
Lateral-patch technology optimizes performance and throughput
 The IonFlux system utilizes a unique microfluidic design to provide automated
cell introduction, trapping, sealing, whole-cell formation and precise current
recording protocols. IonFlux plates are available in 96-well and 384-well formats.
The plates look and handle just like standard well plates. However, the bottom
of the plate has been replaced with a channel network that connects the wells
with a series of tiny channels for cell and compound introduction. The instrument
includes an interface that mates to the plates and provides pneumatic flow control.
Integrated electrodes are inserted into the wells for voltage clamping. Recordings
are taken from 20-cell ensembles to increase robustness and throughput. Pharmacology
is improved by recording the full range of concentrations from the same group
of cells.
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