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| This "Oslo" type brain slice chamber was originally developed for submerged preparations. However, it can also be used for interface preparations by maintaining the fluid level close to the slice's level and ensuring that the lid is in place to increase the humidity and oxygenation. A sloped insert allows room for one or two hippocampal-type slices with low dead volume for expensive drugs etc. |
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| "Haas" type brain slice chambers are interface only -- relying on a thin film of perfusate entering the slice area resting on a piece of lens tissue. The pre-humidified and oxygenated gas passes over the slice, is maintained in high concentration by a lid, and allows the slice to survive for many hours. The BSC2 includes "templates" for two interface perfusion channels or nearly submerged slices. |
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| The BSC3 is available in four and six channel versions, and is a cross between the "Oslo" and "Haas" type BSC1 and BSC2 chambers above. This chamber is intended primarily for pharmacological studies as there is little room for electrodes around four or six channels simultaneously. |
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| The MSC1 microscope stage chamber has been designed for use with fixed-stage upright microscopes. Its profiled contour allows the microscope objective to sweep across the preparation when changing magnification without having to adjust the focus. The specimen and microelectrodes remain par-focal, useful for confocal microscopy combining electrophysiology and precise localization of structures. |
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| The MS3 stage chamber allows interface methods of slice maintenance at room temperature on a compound microscope stage. Constructed from clear acrylic, it can be mounted in close proximity to the microscope condenser optics for optimal visualization. Slices rest in a flat channel with a 20mm central coverslip base. Fluid flows in a diamond-shaped channel above the slice with high oxygen tension above. |
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| The Brain Slice Keeper has been designed to pre-incubate brain slices for many hours prior to transfer into recording chambers. It consists of a pair of closely-fitting upper and lower acrylic rings between which is wedged a sheet of nylon netting. In operation, it is totally immersed in an ACSF-filled Buchner funnel to which a 95% O2, 5% CO2 gas mixture is being supplied. |
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| The Brain Slice
Keeper AM has been designed to pre-incubate brain slices prior
to transfer into recording chambers. The main body of the BSK-AM
comprises a chamber fitted with stainless steel heater and sensor
probes to maintain the desired incubating temperature, regulated
by our temperature controller PTC03. |
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| For in vitro studies of oocytes, organs or aquatic preparations or extracellular, intracellular or patch clamp experiments, this tissue chamber uses a removable insert to adjust the flow rate and submersion level of the prep. The insert has a base of silicone rubber to allow insect pins to be used for holding down preparations. Perfusion fluid flows over the preparation and exits into a circular canal for removal. |
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© 2006-2010 AutoMate Scientific, Inc.
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