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Perfusion Systems

Perfusion Systems

• Unattended solution switching
Avoid vibrations from switching valves by hand. The ValveBank® or ValveLink8.2® controller handles all solution delivery so that you can watch results – not switch stopcocks. Many special features are included for easy perfusion control. Low noise circuitry.

• Increased reproducibility
Valve switching is accurate to 0.01 seconds with programs up to 99 hours long under microprocessor control. Consistent liquid delivery means better data.

• Pinch & PTFE inert and Lee™ Valves
Choose between speed, cost, and ease of cleaning. Several options are available for fittings and reservoirs.

• Manual and external valve control
Flexible design. Easy cleaning and calibration. Slave mode valve operation controlled by your computer, pClamp, Pulse, Acquire, LabView, AxoGraph, etc.


Please see our new How To Configure and Order a Perfusion System for guided assistance!

Valve Choices:

• Pinch Valves for Reduced Maintenance
Easiest valves to clean and switch tubing. Liquids never touch the valves. Switches in 30-50 ms. 1/32” i.d. silicone tube passes through, and is pinched closed by solenoid activation. All AutoMate Scientific valves include an individual indicator LED. Our new aluminum enclosure keeps the valves dry from spills and offers luer lock ports for syringe reservoirs.

• PTFE inert Valves for Fast Switching
Required for fast kinetics applications. Excellent chemical and corrosion resistance. Non-stick surface resists particles and chemical deposits. Switches in less than 10 ms, with 20 μl of dead volume from port to port. Threaded female inlet and outlet ports accept Hose Barb, Luer Lock and Nut & Ferrule fittings (see diagrams next page).

Lee™ Mini Valves for Extremely Fast Switching and Minimal Pressure Pulse
For the most demanding applications AutoMate Scientific offers tiny valves from the Lee Company. Enclosed in our new aluminum box with luer locks for syringe reservoirs, these valves can open and close in 1.5-4 ms with a ValveLink8.2 controller.


Perfusion Systems Include:

Controller, valves, 60 ml syringe reservoirs, 2-way stopcocks, ringstand, 1/16" i.d. tubing and four-, eight- or sixteen-into-one micro-manifold with built-in flow control. Non-pressurized PTFE inert systems include drippers and a reservoir bracket. 5, 15, 35, 60 or 140 ml syringe reservoirs available.

The Economy Pinch Valve System includes ValveLink8 controller, four pinch valves, 35 ml syringes, 2-way stopcocks, ringstand, 1/16” i.d. tubing and four-into-one micro-manifold with built-in flow control
.

Computer Interfacing:

Perfusion systems can be controlled by a computer using data acquisition hardware (i.e., DigiData, ITC-16, or National Instruments board) and software (i.e., pCLAMP, Pulse, or LabView). Both ValveBanks and ValveLinks accept real-time TTL inputs to control valves. Most acquisition software already being used in your experiments can talk to our controllers. AutoMate Scientific offers an optional program called EasyCode® for the Macintosh and PC/ Windows to program ValveBanks (not ValveLinks). This software is used before an experiment – valve sequences are downloaded into the memory of the ValveBank where they are run. An article by AutoMate Scientific can be found in Axon Instrument’s AxoBits 17 newsletter outlining these strategies – accessible on our web site.

"I am writing to tell you how pleased I am with the ValveBank8 Perfusion System. It's great that I can load the reservoirs, press go, and start recording. I can read papers rather than fussing with solutions and switching valves every 10-15 minutes. Since solutions are switched at exactly the same time from one experiment to the next, I have been able to automate my data analysis protocols as well."

Brain Slice Recording
View  Larger Image


Unattended solution delivery using a ValveBank8 AutoPrime Perfusion System (above).

Dr. M. Bruce MacIver,
M.Sc., Ph.D.

Department of Anesthesia
Stanford University
Medical Center

Pinch Valves
Pinch ValveView Larger Image

PTFE inert Valves
PTFE inert ValvesView Larger Image

Lee™ Valves
Lee Valves
View Larger Image


Luer-lock Fittings in PTFE inert Valves
Luer-lock fittings in PTFE inert valves allow direct connection of syringe reservoirs for minimal dead volume.

Luer-lock Fittings in PTFE inert Valves
View Larger Image

PTFE inert Valve Fitting Choices:
Hose Barb
Hose Barb
Standard
Available for 1/8" and 1/16" i.d. soft tubing.

Luer-Lock

Lure-lock
For direct attachment of syringes.
Eliminates extra tubing between reservoirs and valves.
Includes 2-way stopcocks and 35ml syringes.

Nut & Ferrule

Nut & Ferrule
 HPLC-like, screw-in fittings for rigid, small-diameter (1/16" o.d.) tubing.

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