AutoMate Scientific - Ready For Research
Perfusion Systems Perfusion Systems
Pressurized Superfusion Pressurized Superfusion
Temperature Control Temperature Control
SmartSquirt® Microperfusion
Perfusion Chambers Perfusion Chambers
Rodent Fixation Systems
Brain Slice and Tissue Chambers Brain Slice & Tissue Chambers
Customize a Perfusion System
Complete Electrophysiology Rig
Amplifiers and Stimulators Amplifiers & Stimulators
MDC/Axon Instruments Heka Data Acquisition
Heka Data Acquisition Heka Data Acquisition
Siskiyou Mechanicals Siskiyou Mechanicals
Sutter Manipulators Sutter Manipulators
Micropipette Fabrication Micropipette Fabrication
TMC Air Tables TMC Air Tables
MED64 Multi-Electrode Array Multi-electrode Array
Automated Pipette Patch Clamping Automated Patch Clamping
Port-a-Patch® Planar Patch Clamping
Vesicle Prep Pro Bilayer Apparatus
Filter Wheels Optical Instruments
Functionalized Bio-surfaces Functionalized Bio-surface
Main Products Page
Download a Catalog

Objective Heater System Buy NowDownload Brochure
Objective Heater System

Objective Heater System

The Problem

When live-cell imaging requires the use of high numeric aperture lenses, it is necessary to control the temperature of the objective as well. This problem exists because the optical coupling medium (oil, glycerin or water) acts as a thermal coupling medium and draws heat away from the specimen. The thermal mass of a fluid coupled objective is overwhelming when compared to the thermal mass of the cells. Unfortunately microscope manufacturers do not offer temperature controlled objectives; nor do they consider the need for thermal regulation in the design of their objectives. Bioptechs has developed and patented a product that solves this problem for the majority of immersion objectives.

The Solution

To eliminate this thermal gradient, Bioptechs offers a patented Objective Heater® System which includes a heater/sensor and an electronic controller. The heater/sensor is comprised of an adjustable thin-film heating band which surrounds 3/4 of the diameter of the upper region of the central retracting tube of the objective. A surface probe thermal sensor positioned in the gap formed between the ends of the heating band measures the temperature of the objective. This heater/sensor assembly is supported on an adjustable metal mounting to fit objectives ranging from 17 to 35mm in diameter.

The heater loop requires a minimum of 3.5 to 6mm longitudinal, physical contact with the cylindrical objective surface depending on which objective heater is required. In some cases there may be a decorative collar on the objective that must be removed in order to permit adequate surface contact. Depending on the objective, it may be necessary to invert the objective heater and mount the objective upside down for optimal thermal transfer to the lens of the objective. It may also be necessary to isolate the objective from the nosepiece turret for proper objective temperature regulation.

Additional options are available for your objective which can help you heat the objective more efficiently, or perhaps if you would like to cool the objective that option is also available.

Key features of the Bioptechs Objective Heater

Objective Heater System


The Controller is specifically designed to slowly heat the objective over a fifteen minute warm-up period then hold the objective at the set point value within 0.2 degrees C. The Controller operates from ambient to 50 degrees C and has special safety circuitry which utilizes a 0.9 degrees C error window to shut down the controller and sound an alarm if, for any reason, the temperature of the objective deviates after it has reached set point. A user calibration test is also built in to the controller.

Objective diameters 17mm to 38mm
Sensor Thermistor
Temperature range Ambient to 43°C 1.3 Watt
Temperature stability ±0.2°C Typically

Objective Heater System
Thermographic image of a Bioptechs Objective Heater

Objective Heater System
Visible light image of a Bioptechs Objective Heater

Notice the temperature distribution in the following locations: nosepiece, bottom of objective, region above heater band, and top of objective. Power consumption after equilibration is 1.3 watts. The point is that, unless you warm the entire microscope, the microscope will always act as a heat sink with respect to warming the objective. You can expect a small thermal gradient. All objectives have different thermal profiles. Therefore, it is imperative to efficiently transfer heat to the core of the objective and prevent excess heat from radiating from the heater-band. This is exactly what the Bioptechs Objective Heater does!

Not sure what heater to use?

If you have an objective that you are not sure how to heat you have a few options:
  1. You can check this list for your objective and take note of the proper band size to fit your objective.
  2. You can use this reference page to measure your objective and select the appropriate heater yourself.
  3. You can send your objective into Bioptechs to have it sized, photographed and thermally profiled.

If your objective has a decorative collar which prevents an efficient heat transfer, it will need to be removed. You can do this yourself with the aide of a special objective wrench and plier set available from Bioptechs or send in your objective and Bioptechs will remove it for free!

Optional Accessories for the Objective Heater

Item Reason
Wrench and Pliers Used to remove a decorative collar from an objective
8mm Stage Spacer Kit Used to raise the stage of the microscope up 8mm to accommodate for a thermal spacer
Cooling Collar Used to cool the objective

More Information

Demonstration Video (25.3 MB)

Demo Video

PDF thermographic images of Bioptechs products. (340 K)

Special Note:

Although there have been no studies relative to the effects of thermal cycling of objectives between ambient and 37 degrees C, Bioptechs recommends storing the warmed objectives in a constant temperature enclosure when not in use.

The Boekel desktop incubator is well suited for this application as well as a convenient place for temporary storage of specimens or media near your microscope. When using the Bioptechs Objective Heater, Cargille type 37 Immersion oil is highly recommended to optimize the objective's PSF.

This oil is formulated to have a refractive index of 1.515 at 37 degree C (Part #150825). This is an ideal optical coupling medium for in-vitro, live-cell imaging. (not for in-vivo use)
Buy NowDownload Brochure

Top Home Credits Privacy Terms Copyright

©2006-2016 AutoMate Scientific, Inc.