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Closed-System Cooled Chamber FCS2
How it works:
Cells
are plated on a 40mm coverslip and placed into a special FCS2 chamber. This provides a perfuseable laminar flow optical chamber
with user modifiable flow characteristics. The upper glass
element (Microaqueduct slide) is then used to remove heat from
the specimen cavity to be absorbed in the cooled fluid being
circulated in the cavity formed by the addition of an O-ring
sealed window adapter. Gravity or an electric pump is then
used to create a flow of chilled fluid through the heat exchange
cavity in the chamber. The cells remain safely enclosed in
a separate optical enclosure. If more thermal transfer is necessary,
tubing can be installed into the FCS2 base.
Objective
Cooling Collar
The Objective Cooling Collar is an
isolated fluid and thermal transfer
device that attaches to the top of
the objective and provides a means
of attaching coolant lines from a cold
source or chiller bath. The removal
of energy from the objective is more
difficult and less efficient than heating.
Make sure you have enough sub stage
room for the additional diameter required
for the cooling collar and tubing.
Cooling collars are precision machined
to fit specific objectives.
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If you are using high N.A. objectives
at below ambient temperatures it will be necessary to cool
and thermally isolate the objective from the scope. The optional
thermal isolator will prevent condensation from forming on
the lower element of the objective. A gas port is provided
to purge the adapter with dry nitrogen. An anti-reflection
coated window optimized for 340-700nm seals the bottom of the
adapter.
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