Zeitz DMZ Puller
Electrode-Puller for fabrication of intracellular
Glass- Microelectrodes, Injection-Microelectrodes and Patch
electrodes.
Fully automatic polishing of Patch electrodes after
pulling and microprocessor controlled. The puller
is capable of:
• Pulling low resistance electrodes
from thick-walled and thin-walled borosilicate glass
up to 2 mm o.d. The electrodes are sturdy, reliable,
and the instrument fabricates pipettes with high
reproducibility.
• Pulling high-resistance electrodes
up to 100 Mohms.
The DMZ-Universal Puller has the
following fully microprcessor controlled variables:
• Longitudinal distance of pull
• Force of pull, time controlled program
• Force of pull, distance controlled programm
• Time controlled or distance controlled heat application
• Power of heating,
regulated by optical sensor
• Fully automatic fixation and
release of the glass tubing
• Fully automatic polishing
of the two tips
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| Glass tubes are clamped automatically |
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| Fully automatic polishing of
the two tips. |
80 programs can be stored permanently, all variables can
be set independently. One- or two-stage pulling programs,
in which all variables can be set independently for each
stage, with the option of repeating the first stage. The
puller is fully automatic. There is no risk of breaking thin-walled
glass capillaries during fixation because two micromotors
fix the ends of the glass tube by clamping them with precisely
defined force. Even with three-stage-pulling, the two tips
are symmetrical because the position of the glass tube relative
to the heat filament is readjusted after each stage of pulling.
There is no need for air-jet cooling because the duration
of heat application is controlled by programmable approach
and withdrawal of the (U-shaped) heating filament. The program
can include sensing of stiffness of the tubing during heating
which compensates for variations in the wall of the tubing.
Free choice and absolute reproducibility of tip size (down
to 0.1 micrometer), taper and electrode resistance (up to
100MOhms). Especially suitable for pulling patch electrodes
of precisely defined tip diameter (usually 1-30 micrometer).
Such electrodes can also be polished by the puller.
The heating
filament is made of KANTHAL©, a special high-temperature
alloy. Although KANTHAL© is extremely robust and durable,
the extremely precise control of filament heat, a prerequisite
for the puller’s exceptional pipette reproducibility,
requires that the filament heating time constant be as low
as possible, Since this limits the lifetime of the filament,
it is important that the filament be replaced in good time
so as to maintain the pipette reproducibility. Compared to
other pullers, the range of electrode tip sizes and shapes
is relatively independent of the precise shape and position
of the heating filament. Therefore repeated readjustment
of the heating filament is not necessary.
Because of the
excellent reproducibility of the electrodes (even over months)
the DMZ Universal puller is suitable for pulling electrodes
immediately before use. Prefabrication of electrodes before
the experiment is no longer required.
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