Brain Slice Keeper and Holder
- Slices maintained for many hours prior to transfer to recording chamber
- Simple to set up and maintain
- Quick-change nylon net
The Brain Slice Keeper has been designed to pre-incubate brain slices prior to transfer into recording chambers. It consists of a pair of closely fitting acrylic rings, upper and lower, between which is wedged a sheet of nylon netting. In operation, the BSK1 is totally immersed in an ACSF-filled purpose designed holding vessel (BSKV) into which a 95% O2, 5% CO2 gas mixture is supplied with a ceramic bubbler. The bubbles rising from the base of the BSKV saturate the ACSF and provide constant circulation of medium to the slices which rest on the nylon net. Since the lower ring of the BSK1 carries a deflector shield, bubbles are prevented from being trapped under the slices and ensure continuous circulation of medium. Slices remain viable for many hours in these conditions.
The BSK1 together with the BSKV holding vessel can easily be placed into a water bath for regulating the incubating temperature as desired. Alternatively a standard Buchner funnel with 40 to 60 micron porosity sintered glass membrane can be used instead of the BSKV. The BSKV now includes a needle valve for adjusting gas flow.
References – Brain Slice Keeper
- Whole-Cell Patch-Clamp Electrophysiology to Study Ionotropic Glutamatergic Receptors and Their Roles in Addiction Jonna M. Leyrer-Jackson, M. Foster Olive, Cassandra D. GipsonGlutamate Receptors, pp 107-135, 02 February 2019
- The Nicotinic Acetylcholine Receptor {alpha}5 Subunit Plays a Key Role in Attention Circuitry and Accuracy Craig D. C. Bailey, Mariella De Biasi, Paul J. Fletcher, and Evelyn K. LambeJ. Neurosci. 2010; 30(27): p. 9241-9252
- The abnormal firing of Purkinje cells in the knockin mouse model of DYT1 dystonia Yuning Liu, Hong Xing, Bradley J. Wilkes, Fumiaki Yokoi, Huanxin Chen, David E. Vaillancourt, Yuqing LiBrain Research Bulletin, September 2020
- Studies on CRMP2 SUMOylation-deficient transgenic mice identify sex-specific NaV1.7 regulation in the pathogenesis of chronic neuropathic pain Aubin Moutal, Song Cai, Jie Yu, Harrison J. Stratton, Aude Chefdeville, Kimberly Gomez, Dongzhi Ran, Cynthia L. Madura, Lisa Boinon, Maira Soto, Yuan Zhou, Zhiming Shan, Lindsey A. Chew, Kathleen E. Rodgers, Rajesh KhannaBiorxiv, April 2020
- Preparation of Rat Organotypic Hippocampal Slice Cultures Using the Membrane-Interface Method Timothy W. Church, Matthew G. GoldPatch Clamp Electrophysiology. Methods in Molecular Biology, vol 2188. Humana, New York, NY. October 2020
- Preparing Viable Hippocampal Slices from Adult Mice for the Study of Sharp Wave-ripples Liu, L., Zhou, X. and Wu, J.Bio-protocol 10(19): e3771, October 2020
- Preparation of Acute Brain Slices Using an Optimized N-Methyl-D-glucamine Protective Recovery Method Ting, J. T., Lee, B. R., Chong, P., Soler-Llavina, G., Cobbs, C., Koch, C., Zeng, H., Lein, E.Jove, Issue 132, e53825, Feb 2018
- Opening of KATP Channel Regulates Tonic Currents From Pyramidal Neurons in Rat Brain Zhongxia Li, Jiangping Wang, Huimin Yu and Kewen JiangCanadian Journal of Neurological Sciences, Vol. 44, Issue 6 November 2017, pp. 718-725
- Neocortical Microdissection at Columnar and Laminar Resolution for Molecular Interrogation Koen Kole, Tansu CelikelCurrent Protocols in Neuroscience, Vol. 86, Issue 1, January 2019
- Impaired Cholinergic Excitation of Prefrontal Attention Circuitry in the TgCRND8 Model of Alzheimer's Disease Eliane Proulx, Paul Fraser, JoAnne McLaurin, and Evelyn K. LambeJ. Neurosci. 2015; 35(37): p. 12779-12791
- h-Channels Contribute to Divergent Intrinsic Membrane Properties of Supragranular Pyramidal Neurons in Human versus Mouse Cerebral Cortex Brian E. Kalmbach, Anatoly Buchin, Brian Long, Jennie Close, Anirban Nandi, Jeremy A.Miller, Trygve E. Bakken, Rebecca D.Hodge, Peter Chong, Rebecca de Frates, Kael Dai, Zoe Maltzer, Philip R. Nicovich, C. Dirk, Keene, Daniel L. Silbergeld, Ryder P. Gwinn, Charles Cobbs, Andrew L.Ko, Jonathan T.TingNeuron, Vol. 100, Issue 5, Pages 1194-1208.e5, December 2018
- Developmental Excitation of Corticothalamic Neurons by Nicotinic Acetylcholine Receptors Sameera M. Kassam, Patrick M. Herman, Nathalie M. Goodfellow, Nyresa C. Alves, and Evelyn K. LambeJ. Neurosci. 2008; 28(35): p. 8756-8764
- Betaine in the Brain: Characterization of Betaine Uptake, its Influence on Other Osmolytes and its Potential Role in Neuroprotection from Osmotic Leena S. Knight, Quinn Piibe, Ian Lambie, Christopher Perkins, Paul H. YanceyNeurochemical Research, Vol. 42, Issue 12, pp 3490–3503, December 2017
- ARCAgRP/NPY Neuron Activity Is Required for Acute Exercise-Induced Food Intake in Un-Trained Mice Wyatt Bunner, Taylor Landry, Brenton Thomas Laing, Peixin Li, Zhijian Rao, Yuan Yuan and Hu HuangFront. Physiol., May 2020
- Apamin Improves Prefrontal Nicotinic Impairment in Mouse Model of Alzheimer's Disease É Proulx, S K Power, D K Oliver, D Sargin, J McLaurin, E K LambeCerebral Cortex, bhz107, June 2019
- Alterations of the electrophysiological properties from cortical layer 5 pyramidal neurons in temporary rapamycin-treated rodent brain slices Keming Rena, Lijuan Chen, Guoxia Sheng, Jiangping Wang, Xiaoming Jin, Kewen JiangNeuroscience Letters, Vol. 612, 26 January 2016, Pages 80-86
- AgRP/NPY Neuron Excitability Is Modulated by Metabotropic Glutamate Receptor 1 During Fasting Brenton T. Laing, Peixin Li, Cameron A. Schmidt, Wyatt Bunner, Yuan Yuan, Taylor Landry, Amber Prete, Joseph M. McClung, and Hu HuangFront Cell Neurosci. 2018; 12: 276.
- A robust ex vivo experimental platform for molecular-genetic dissection of adult human neocortical cell types and circuits Jonathan T. Ting, Brian Kalmbach, Peter Chong, Rebecca de Frates, C. Dirk Keene, Ryder P. Gwinn, Charles Cobbs, Andrew L. Ko, Jeffrey G. Ojemann, Richard G. Ellenbogen, Christof Koch & Ed LeinNature Scientific Reports, Vol. 8, Article number: 8407 (2018)