Brain Slice Keeper – BSK AM
- Pre-incubate slices at controlled temperatures prior to transfer to recording chamber
- Inlet and outlet ports used to supply ACSF to Alpha MED Sciences multi-electrode array probes
- Simple to set up and maintain, modular design allows quick cleaning and assembly
- Quick-change nylon net
The Brain Slice Keeper AM has been designed to pre-incubate brain slices prior to transfer into recording chambers. The main body of the BSK-AM comprises a chamber fitted with stainless steel heater and sensor probes to maintain the desired incubating temperature, regulated by our temperature controller PTC03. Within the body of the chamber is a holder for resting the slices, it consists of a pair of closely fitting acrylic rings, upper and lower, between which is wedged a removable sheet of nylon netting. In operation, the BSK-AM is filled with ACSF so as to totally immerse the net and ring.
A ceramic bubbler to one side is supplied with 95% O2/ 5%CO2 gas mixture to both saturate the ACSF and to produce circulation of the ACSF from the top downwards on to the net surface on which brain slices are placed. The circulation also provides for even heat distribution and feedback control from the heating element and sensor elements below. A deflector shield between the bubbler and the base of the nylon net prevents bubbles from being trapped under the slices and ensure continuous circulation of medium. Slices remain viable for many hours in these conditions. The BSK-AM fluid volume is typically 150ml. A close fitting lid with a sealing ring carries two stainless tubes into the base of the chamber. These tubes can be used to provide ASCF to the Alpha MED Sciences Perfusion Cap for their multi-electrode array probes. A third venting tube carries humidified oxygen which can also be used to supply the Alpha MED Sciences cap for interface slice applications.
Alpha MED Sciences, MED Probes and MED Perfusion Caps are the products and Trade Names and Trade Marks of Alpha MED Sciences products. Visit them at: www.med64.com
Click here for a recent publication using this chamber.
References – Brain Slice Keeper
- Whole-Cell Patch-Clamp Electrophysiology to Study Ionotropic Glutamatergic Receptors and Their Roles in Addiction Glutamate Receptors, pp 107-135, 02 February 2019Jonna M. Leyrer-Jackson, M. Foster Olive, Cassandra D. Gipson
- The Nicotinic Acetylcholine Receptor {alpha}5 Subunit Plays a Key Role in Attention Circuitry and Accuracy J. Neurosci. 2010; 30(27): p. 9241-9252Craig D. C. Bailey, Mariella De Biasi, Paul J. Fletcher, and Evelyn K. Lambe
- The abnormal firing of Purkinje cells in the knockin mouse model of DYT1 dystonia Brain Research Bulletin, September 2020Yuning Liu, Hong Xing, Bradley J. Wilkes, Fumiaki Yokoi, Huanxin Chen, David E. Vaillancourt, Yuqing Li
- Studies on CRMP2 SUMOylation-deficient transgenic mice identify sex-specific NaV1.7 regulation in the pathogenesis of chronic neuropathic pain Biorxiv, April 2020Aubin Moutal, Song Cai, Jie Yu, Harrison J. Stratton, Aude Chefdeville, Kimberly Gomez, Dongzhi Ran, Cynthia L. Madura, Lisa Boinon, Maira Soto, Yuan Zhou, Zhiming Shan, Lindsey A. Chew, Kathleen E. Rodgers, Rajesh Khanna
- Preparation of Rat Organotypic Hippocampal Slice Cultures Using the Membrane-Interface Method Patch Clamp Electrophysiology. Methods in Molecular Biology, vol 2188. Humana, New York, NY. October 2020Timothy W. Church, Matthew G. Gold
- Preparing Viable Hippocampal Slices from Adult Mice for the Study of Sharp Wave-ripples Bio-protocol 10(19): e3771, October 2020Liu, L., Zhou, X. and Wu, J.
- Preparation of Acute Brain Slices Using an Optimized N-Methyl-D-glucamine Protective Recovery Method Jove, Issue 132, e53825, Feb 2018Ting, J. T., Lee, B. R., Chong, P., Soler-Llavina, G., Cobbs, C., Koch, C., Zeng, H., Lein, E.
- Opening of KATP Channel Regulates Tonic Currents From Pyramidal Neurons in Rat Brain Canadian Journal of Neurological Sciences, Vol. 44, Issue 6 November 2017, pp. 718-725Zhongxia Li, Jiangping Wang, Huimin Yu and Kewen Jiang
- Neocortical Microdissection at Columnar and Laminar Resolution for Molecular Interrogation Current Protocols in Neuroscience, Vol. 86, Issue 1, January 2019Koen Kole, Tansu Celikel
- Impaired Cholinergic Excitation of Prefrontal Attention Circuitry in the TgCRND8 Model of Alzheimer's Disease J. Neurosci. 2015; 35(37): p. 12779-12791Eliane Proulx, Paul Fraser, JoAnne McLaurin, and Evelyn K. Lambe
- h-Channels Contribute to Divergent Intrinsic Membrane Properties of Supragranular Pyramidal Neurons in Human versus Mouse Cerebral Cortex Neuron, Vol. 100, Issue 5, Pages 1194-1208.e5, December 2018Brian E. Kalmbach, Anatoly Buchin, Brian Long, Jennie Close, Anirban Nandi, Jeremy A.Miller, Trygve E. Bakken, Rebecca D.Hodge, Peter Chong, Rebecca de Frates, Kael Dai, Zoe Maltzer, Philip R. Nicovich, C. Dirk, Keene, Daniel L. Silbergeld, Ryder P. Gwinn, Charles Cobbs, Andrew L.Ko, Jonathan T.Ting
- Developmental Excitation of Corticothalamic Neurons by Nicotinic Acetylcholine Receptors J. Neurosci. 2008; 28(35): p. 8756-8764Sameera M. Kassam, Patrick M. Herman, Nathalie M. Goodfellow, Nyresa C. Alves, and Evelyn K. Lambe
- Betaine in the Brain: Characterization of Betaine Uptake, its Influence on Other Osmolytes and its Potential Role in Neuroprotection from Osmotic Neurochemical Research, Vol. 42, Issue 12, pp 3490–3503, December 2017Leena S. Knight, Quinn Piibe, Ian Lambie, Christopher Perkins, Paul H. Yancey
- ARCAgRP/NPY Neuron Activity Is Required for Acute Exercise-Induced Food Intake in Un-Trained Mice Front. Physiol., May 2020Wyatt Bunner, Taylor Landry, Brenton Thomas Laing, Peixin Li, Zhijian Rao, Yuan Yuan and Hu Huang
- Apamin Improves Prefrontal Nicotinic Impairment in Mouse Model of Alzheimer's Disease Cerebral Cortex, bhz107, June 2019É Proulx, S K Power, D K Oliver, D Sargin, J McLaurin, E K Lambe
- Alterations of the electrophysiological properties from cortical layer 5 pyramidal neurons in temporary rapamycin-treated rodent brain slices Neuroscience Letters, Vol. 612, 26 January 2016, Pages 80-86Keming Rena, Lijuan Chen, Guoxia Sheng, Jiangping Wang, Xiaoming Jin, Kewen Jiang
- AgRP/NPY Neuron Excitability Is Modulated by Metabotropic Glutamate Receptor 1 During Fasting Front Cell Neurosci. 2018; 12: 276.Brenton T. Laing, Peixin Li, Cameron A. Schmidt, Wyatt Bunner, Yuan Yuan, Taylor Landry, Amber Prete, Joseph M. McClung, and Hu Huang
- A robust ex vivo experimental platform for molecular-genetic dissection of adult human neocortical cell types and circuits Nature Scientific Reports, Vol. 8, Article number: 8407 (2018)Jonathan T. Ting, Brian Kalmbach, Peter Chong, Rebecca de Frates, C. Dirk Keene, Ryder P. Gwinn, Charles Cobbs, Andrew L. Ko, Jeffrey G. Ojemann, Richard G. Ellenbogen, Christof Koch & Ed Lein