Precision engineered, for scientific investigations.
Brain Slice Chamber: Interface and Submerged
The BSC1 chamber design is similar to the original “Oslo chamber” (Langmoen & Andersen 1981) with later modifications (Dhanjal & Sears 1980, Alger et al, 1984) to allow both interface and submerged methods of slice maintenance.
“Submerged” and “Interface” methods of slice maintenance with same chamber
This chamber allows adaptation to either method of slice maintenance simply by altering the fluid level by means of a screw adjustment device on the chamber.
Slices supported on removable insert adaptable to your requirements
A standard insert having a nylon net is supplied with the chamber for submerged and interface methods. This insert can be modified or custom made to accommodate other preparations eg. adult spinal cord (Dhanjal & Sears 1980, Alger et al, 1984) and Cerebellar slices (Crepel et al 1981).
Proportional Temperature Controller (PTC03) with low noise performance
* Available separately
The temperature is controlled by a proportional control heating unit, the PTC03 which provides smooth DC controlled power to the heater element incorporated into the chamber. Please order this item separately.
The chamber is constructed from clear acrylic having a diameter of 100mm, height is 75mm and the base plate is 130mm in diameter. The slices rest on a nylon net fixed on to a removable insert, located in the centre of the chamber. Pre-oxygenated medium enters the main body of the chamber through a fine bore tube which spirals in the heated distilled water in the lower part of the chamber and enters the upper part of the chamber via a bubble trap. Alternatively a secondary pathway is built in such that the bubble trap is by-passed and the fluid arranged to enter above the surface of the slice by use of a fine-bore stainless steel tube. Depending on whether submerged or interface type preparations are required, the height of the perfusion fluid is adjusted at the exit well by means of a needle on a screw mechanism. In addition, the net-type removable insert can be replaced by an optional insert having a sloping grove.
The slices then rest on a piece of lens tissue while being perfused from above via the fine stainless steel tube. In the case of interface preparations, the high oxygen tension is maintained by bubbling a 95% oxygen, 5% carbon dioxide gas mixture through a ceramic bubbler located in the lower heated part of the chamber. This moistened and warmed gas mixture enters the upper part of the chamber via “port holes” and is then deflected by a profiled lid across and downwards towards the centrally located slice preparation. The temperature in the upper chamber is maintained by ensuring that the medium and moistened gas mixture enter at the required temperature. This is dependent on the temperature of the lower chamber body which is warmed by a heating element controlled by the Proportional Temperature Controller, PTC03. An optional monitor sensor allows the upper chamber temperature to be checked when required.
References – Brain Slice Chambers
- Two forms of CX3CL1 display differential activity and rescue cognitive deficits in CX3CL1 knockout mice Journal of Neuroinflammation Volume 17, Article# 157, May 2020Aimee N. Winter, Meena S. Subbarayan, Bethany Grimmig, Jason A. Weesner, Lauren Moss, Melinda Peters, Edwin Weeber, Kevin Nash & Paula C. Bickford
- Toll‐like Receptor 4 Signaling in Neurons Enhances Calcium‐Permeable AMPA Receptor Currents and Drives Post‐Traumatic Epileptogenesis Annals of Neurology, Volume 87, Issue 4, February 2020Akshata A. Korgaonkar PhD, Ying Li PhD, Dipika Sekhar MS, Deepak Subramanian PhD, Jenieve Guevarra MBS, Bogumila Swietek MS, Alexandra Pallottie PhD
- Systemic or Forebrain Neuron-Specific Deficiency of Geranylgeranyltransferase-1 Impairs Synaptic Plasticity and Reduces Dendritic Spine Density Neuroscience, Vol. 373, 1 March 2018, Pages 207-217David Hottman, Shaowu Cheng, Andrea Gram, Kyle LeBlanc, Li-Lian Yuan, Ling Li
- nrf2-deficiency-exacerbates-obesity-induced-oxidative-stress-neurovascular-dysfunction-blood-brain-barrier-disruption-neuroinflammation-amyloidogenic-gene-expression-and-cognitive-decline The Journals of Gerontology: Series A, Vol. 73, Issue 7, pp 853–863, July 2018Stefano Tarantini, PhD, M Noa Valcarcel-Ares, PhD, Andriy Yabluchanskiy, MD, PhD, Zsuzsanna Tucsek, PhD, Peter Hertelendy, MD, Tamas Kiss, MD, Tripti Gautam, MS, Xin A Zhang, MD, PhD, William E Sonntag, PhD, Rafael de Cabo, PhD, Eszter Farkas, PhD, Michael H Elliott, PhD, Michael T Kinter, PhD, Ferenc Deak, MD, PhD, Zoltan Ungvari, MD, PhD
- Neuronal TLR4 signaling enhances AMPA currents and drives posttraumatic epileptogenesis BioRxiv, May 2019Akshata A. Korgaonkar, Ying Li, Dipika Sekhar, Deepak Subramanian, Jenieve Guevarra, Bogumila Swietek, Alexandra Pallottie, Sukwinder Singh, Kruthi Kella, Stella Elkabes, Vijayalakshmi Santhakumar
- Mitigation of Carbon Nanotube Neurosensor Induced Transcriptomic and Morphological Changes in Mouse Microglia with Surface Passivation ACS Nano 2020, September 2020Darwin Yang, Sarah J. Yang, Jackson Travis Del Bonis-O’Donnell, Rebecca L. Pinals, and Markita P. Landry
- Imaging striatal dopamine release using a nongenetically encoded near infrared fluorescent catecholamine nanosensor Science Advances, Vol. 5, no. 7, July 2019Abraham G. Beyene, Kristen Delevich, Jackson Travis Del Bonis-O’Donnell, David J. Piekarski, Wan Chen Lin, A. Wren Thomas, Sarah J. Yang, Polina Kosillo, Darwin Yang, George S. Prounis, Linda Wilbrecht and Markita P. Landry
- Hypertension-induced synapse loss and impairment in synaptic plasticity in the mouse hippocampus mimics the aging phenotype: implications for the pathogenesis of vascular cognitive impairment GeroScience, Vol. 39, Issue 4, pp 385–406, August 2017Zsuzsanna Tucsek, M. Noa Valcarcel-Ares, Stefano Tarantini, Andriy Yabluchanskiy, Gábor Fülöp, Tripti Gautam, Albert Orock, Anna Csiszar, Ferenc Deak, Zoltan Ungvari
- High-throughput evolution of near-infrared serotonin nanosensors Science Advances, Vol. 5, no. 12, December 2019Sanghwa Jeong, Darwin Yang, Abraham G. Beyene, Jackson Travis Del Bonis-O’Donnell, Anneliese M. M. Gest, Nicole Navarro, Xiaoqi Sun and Markita P. Landry
- Distinct cellular mediators drive the Janus Faces of Toll-like Receptor 4 regulation of network excitability which impacts working memory performance after brain Injury BioRxiv, September 2019Akshata A. Korgaonkar, Ying Li, Jenieve Guevarra, Kevin C H Pang, Vijayalakshmi Santhakumar
- Differential Modulation of Spontaneous and Evoked Thalamocortical Network Activity by Acetylcholine Level In Vitro J. Neurosci. 2013; 33(45): p. 17951-17966Jason C. Wester and Diego Contreras
- Decrease in Tonic Inhibition Contributes to Increase in Dentate Semilunar Granule Cell Excitability after Brain Injury Journal of Neuroscience 15 February 2012, 32 (7) 2523-2537Akshay Gupta, Fatima S. Elgammal, Archana Proddutur, Samik Shah and Vijayalakshmi Santhakumar
- Deep brain stimulation of the ventroanterior and ventrolateral thalamus improves motor function in a rat model of Parkinson's disease Experimental Neurology, Vol. 317, July 2019, p. 155-167Heidi R. Tucker, Emily Mahoney, Ashok Chhetri, Kristen Unger, Gianna Mamone, Gabrielle Kim, Aliyah Audil, Benjamin Moolick, Eric S. Molhob, Julie G. Pilitsis, Damian S. Shin
- Autism-Associated Insertion Mutation (InsG) of Shank3 Exon 21 Causes Impaired Synaptic Transmission and Behavioral Deficits J. Neurosci. 2015; 35(26): p. 9648-9665Haley E. Speed, Mehreen Kouser, Zhong Xuan, Jeremy M. Reimers, Christine F. Ochoa, Natasha Gupta, Shunan Liu, and Craig M. Powell
- A Peptidergic Circuit Links the Circadian Clock to Locomotor Activity Current Biology, Vol. 27, Issue 13, 10 July 2017, Pages 1915-1927.e5Anna N. King, Annika F. Barber, Amelia E. Smith, Austin P. Dreyer, Divya Sitaraman, Michael N. Nitabach, Daniel J. Cavanaugh, Amita Sehga