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If you would like quotations on several system configurations, please submit multiple copies of this form, and let us know it’s not an accident in the comments box near the bottom. Don’t worry, you are NOT placing an order – only getting a quote.

Please see our new Perfusion System Configuration Guide for additional help!

Depending on component choices, AutoMate Scientific perfusion systems include: Controller, valves, reservoirs, ringstand, 1/16″ i.d tubing and four-, eight-, or sixteen-into-one micro-manifold with flow regulator. Non-pressurized PTFE inert systems include drippers and a reservoir bracket.

  • Prices shown are U.S. prices for comparison only.
  • International prices are at least 15% higher.
  • Prices subject to change without notice.
  • Questions are optional.

Perfusion System Configuration Options

    Number of channels (number of valves & liquids)
    Example prices shown are for a ValveLink® Pinch Valve System

    4 Channels - $1,7628 Channels - $3,06216 Channels - $5,817

    Valve controller
    Example prices shown are for an 8-Channel Pinch System

    TTL, computer, manual control ValveLink® - $3,062TTL, computer, manual and programmable ValveBank® - $3,607

    Valve type
    Example prices shown are for an 8-Channel ValveLink System

    Inexpensive, slow (10-20ms open/close time) Pinch Valves - $3,062Fast (8-10ms) PTFE inert Valves - $3,782Very Fast (1.5-4ms) Lee® Mini Valves - $3,852SmartSquirt® Micro-Perfusion System (skip the next two questions) - $3,467

    Low-noise valve grounding package
    $65 per 4 valves. Required for Europe and suggested for electrophysiology


    Pressurized system or gravity-fed reservoirs
    8 channels

    Gravity 140ml syringe reservoirs - $0Gravity 60ml syringes - $0Gravity 35ml syringes - $0Gravity 15ml syringes - $0Pressurized 60ml syringes - $695Pressurized 50ml screw-cap reservoirs - $695Pressurized 35ml syringes - $695Pressurized Glass 35ml for volatiles - $935

    Temperature control

    Room TemperatureThermoClamp® - Heated Perfusion Pencil (inline heater) with controller - $1,715BubbleStop® Syringe Heater (gravity) - $1,035BubbleStop® Syringe Heater (pressurized) - $1,450Cool Solutions - Heated and Cooled inline Peltier with controller - $4,417

    QuickStage HEATED perfusion chambers
    Includes ThermoClamp temperature controller

    Heated 12 mm diamond (these also accept 24 x 40 mm coverslips) - $1,872Heated 5 mm diamond - $1,872Heated large slice - $1,872Heated 35 mm Petri dish holder (Choose dish model below) - $1,947

      NuncWillCoFalcon/In Vitro 35 mmCorning-MattekIn Vitro 29 mm

    Please specify microscope model, upright/inverted, and stage:

    QuickStage UNHEATED perfusion chambers

    Unheated horizontal chamber (also takes 22 mm square and 13 mm round coverslips) - $437Unheated 35 mm Petri dish holder (Choose dish model below) - $527

      NuncWillCoFalcon/In Vitro 35 mmCorning-MattekIn Vitro 29 mm

    Gas bubblers for slice oxygenation

    Add gas bubblers to any gravity system, without regulator - $115Add gas bubblers to any gravity system - with regulator - $340Oxygen8® bubbled AND pressurized 35 or 60 ml syringes - $1,936

    Do you need our interface cable for connecting your valve controller to interface hardware like the DigiData or ITC interface?

    4-BNC cable to Axon Digidata 1320 - 1322A - $958-BNC cable to Digidata 1440 & 1550, National Instruments, others - $180Cable to Heka EPC-10 / ITC-1600 - $99Cable to Heka EPC-10 USB - $99USB A-B cable for ValveLink8.2 - $9No Thanks

    Do you need brackets for mounting your valve controller in a 19" rack?

    Yes - $80No

    If you chose the ValveBank® controller, would you like our EasyCode® software to help program it before experiments?

    No thanksEasyCode for PC (Windows 98SE, 2000, XP, 7, 8, 10) - $249

    Depending on component choices, AutoMate Scientific perfusion systems include:
    Controller, valves, reservoirs, ringstand, 1/16" i.d tubing and four-, eight-, or sixteen-into-one micro-manifold with flow regulator. Non-pressurized PTFE inert systems include drippers and a reservoir bracket.

    Perfusion Pencil®

    4-channel Pencil (perfusion manifold) - $2708-channel Pencil - $27016-channel Pencil for fast, local drug application (one tip included) - $395

      Additional tips for Perfusion Pencil, SmartSquirt and ThermoClamp (quantity/$35 ea.):

      100µm i.d.

      250µm i.d.

      360µm i.d.

    Sub-millisecond piezo solution switcher

    Yes - $10,750No, theta glass piezo switcher

    Fast exchange, open Oocyte chambers

    80 mm - $25090 mm - $250100 mm - $250108 mm - $250110 mm - $250120 mm - $250

    Petri dish perfusion chamber insert

    Nunc, default 35mm dish - $195Corning - $195Falcon - $195

    Extra tubing
    Minimum, 10ft.

    1/16" i.d. Tygon, $.52 per foot

    1/16" o.d. PTFE inert, $2.20 per foot

    1/32" i.d. Silicone pinch valve, $3.90 per foot

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    Determines the number of liquids that can be controlled. 4-channel ValveLink systems actually come with a ValveLink8.2 controller (we don’t make a ValveLink4), so they can easily be expanded later by adding more valves. 16-channel systems come with two controllers connected electronically to act as one unit. We can daisy-chain a perfusion system with up to 80 channels. Please contact us.

    ValveLinks are less expensive than AutoMate Scientific’s ValveBank controllers and include manual pushbuttons, digital/TTL, analog and USB inputs to control valves. They are designed for easy control by your data acquisition software. ValveBanks include manual control, digital inputs, plus they can be programmed through their keypad and LCD screen or EasyCode® software from a Macintosh or PC-Windows. ValveBanks run user valve sequence ‘programs’ without a computer, and include eight programmable digital outputs for control of external devices. Entire ValveBank programs can also be triggered by a single TTL pulse.

    See a comparison of ValveLink and ValveBank controllers.

    Pinch valves – 1/32″ i.d. silicon tube passes through, and is pinched closed by the valve. No liquid comes into contact with the valve. Easiest valves to clean and switch tubing; however, switching times are increased to 10-20ms. Less expensive.

    PTFE-inert valves – Excellent chemical and corrosion resistance. Non-stick surface resists particle and chemical deposits. Open and close in approximately 8-10 ms. More expensive.

    SmartSquirt® – Pneumatic valves use air pressure to push precious solutions from small cryo tube reservoirs located near the prep.

    More about SmartSquirt

    Priced per set of four valves. An extra grounding wire attached to all valves extending back to the controller. This item is required for CE conformity in Europe, and highly recommended for all electrophysiology.

    It must be ordered when purchasing the valves – it cannot be added later.

    For anyone who needs:

    • Faster Switching
    • Steady flow-rate
    • Small diameter pipette delivery
    • Microliter fine delivery
    • Microinjecting

    Pressurization will help give more consistent flow rates with any perfusion system. It will also help overcome many problems associated with bubbles in the tubing, although this should really be accomplished by warming your solutions above room temperature (to outgas them) BEFORE your experiments. AutoMate Scientific’s pressure upgrade was primarily designed for use with the PerfusionPencil – our multi-barrel manifold tip. It is difficult to obtain liquid flow through our 250 µm tip with just gravity, and impossible through the 100 µm tip (see chart below). Several psi of air pressure results in ideal flow rates and very fast switching times through the PerfusionPencil.

    • Can be added to any new or existing perfusion rig – from AutoMate Scientific, any 3rd party manufacturer, even homemade!
    • Connect to house air, or compressor (30 to 100 psi).
    • Precision regulator delivers zero to 10 psi.
    • Available with four, eight or sixteen channels.
    • Syringe reservoirs may also be placed in a water bath.
    • Sturdy stainless steel construction.
    • Includes 5 micro bowl filter and gauge.
    • Elegant design allows individual air control and easy refilling.

    Build Your Own - Superfusion

    Our valve controllers use DB-9, DB-15, and DB-25 connectors respectively for their digital inputs (similar connectors to serial ports on computers). Some data acquisition hardware (i.e. DigiData, ITC-16, etc.) or computer interface hardware (i.e. National Instruments) use BNC connectors for their digital outputs. Others offer custom digital ports. AutoMate offers a variety of special cables pre-made to connect the valve controllers to digital outputs. Or, if you plan on controlling valves from your computer, you can make such a cable yourself.

    Program your valve and TTL on-off sequences with a Macintosh or PC-Windows compatible computer using graphic ‘click-and-drag’ timebars. Save and load unlimited programs to disk, print out program listings, then download your programs into the ValveBank’s memory in one simple step.

    More about EasyCode®

    • 4, 8 or 16 micro-bore tubes to 1 outflow
    • For single-cell and patch delivery
    • Rapid solution change
    • Very small dead volume
    • Vacuum cross-contamination prevention (use one tube to vacuum the tip volume clear between solution changes)
    • For mounting on any micromanipulator

    Build Your Own, Perfusion Pencil

    A Xenopus oocyte perfusion chamber for use in automated and unattended experiments. One of the major characteristics of this chamber is a small working volume (~20 µl), which allows for fast solution exchange.

    More about Oocyte Chamber

    Build Your Own - Oocyte

    Perfuse cells right in your Petri Dish. Cells cultured in Petri dishes are a popular research tool used in numerous applications, including patch clamping and intracellular ion probe imaging. True perfusion (continuous inflow and outflow) of solutions can be difficult to configure. This forces scientists to plate cells on cover slips for placement into specially designed perfusion chambers. However, such transfer is a time consuming process which introduces the potential for contamination plus additional expense.

    The PCP-1 chamber was designed by scientists after years of patch clamp research to overcome this problem. Perfuse cells right in your Petri dish with any perfusion system and an optional PTFE-Inert manifold (sold separately). Ideal for inverted microscopy using optically clear Petri dishes. Adjustable metal suction tube included. Dimensions: 35mm, outside diameter x 20mm tall.

    More about Petri Chambers

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