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Build Your Own Perfusion System

Home|Build Your Own Perfusion System

For a quote, fill out the form below

If you would like quotations on several system configurations, please submit multiple copies of this form, and let us know it’s not an accident in the comments box near the bottom. Don’t worry, you are NOT placing an order – only getting a quote.

Please see our new Perfusion System Configuration Guide for additional help!

Depending on component choices, AutoMate Scientific perfusion systems include: Controller, valves, reservoirs, ringstand, 1/16″ i.d tubing and four-, eight-, or sixteen-into-one micro-manifold with flow regulator. Non-pressurized PTFE inert systems include drippers and a reservoir bracket.

  • Prices shown are U.S. prices for comparison only.
  • International prices are at least 15% higher.
  • Prices subject to change without notice.
  • Questions are optional.

Perfusion System Configuration Options

Number of channels (number of valves & liquids)
Example prices shown are for a ValveLink® Pinch Valve System

4 Channels - $1,5628 Channels - $2,66216 Channels - $5,017

Valve controller
Example prices shown are for an 8-Channel Pinch System

TTL, computer, manual control ValveLink® - $2,662TTL, computer, manual and programmable ValveBank® - $3,207

Valve type
Example prices shown are for an 8-Channel ValveLink System

Inexpensive, slow (10-20ms open/close time) Pinch Valves - $2,662Fast (8-10ms) PTFE inert Valves - $3,462Very Fast (1.5-4ms) Lee® Mini Valves - $3,852SmartSquirt® Micro-Perfusion System (skip the next two questions) - $3,324

Low-noise valve grounding package
$55 per 4 valves. Required for Europe and suggested for electrophysiology

YesNo

Pressurized system or gravity-fed reservoirs
8 channels

Gravity 140ml syringe reservoirs - $0Gravity 60ml syringes - $0Gravity 35ml syringes - $0Gravity 15ml syringes - $0Pressurized 60ml syringes - $495Pressurized 50ml screw-cap reservoirs - $495Pressurized 35ml syringes - $495Pressurized Glass 35ml for volatiles - $1,090

Temperature control

Room TemperatureThermoClamp® - Heated Perfusion Pencil (inline heater) with controller - $1,920BubbleStop® Syringe Heater - $900 / 1,300Cool Solutions - Heated and Cooled inline Peltier with controller - $4,116

QuickStage HEATED perfusion chambers
Includes ThermoClamp temperature controller

Heated 12 mm diamond (these also accept 24 x 40 mm coverslips) - $2,067Heated 5 mm diamond - $2,067Heated large slice - $2,067Heated 35 mm Petri dish holder (Choose dish model below) - $2,142

    NuncWillCoFalcon/In Vitro 35 mmCorning-MattekIn Vitro 29 mm

Please specify microscope model, upright/inverted, and stage:

QuickStage UNHEATED perfusion chambers

Unheated horizontal chamber (also takes 22 mm square and 13 mm round coverslips) - $417+Unheated 35 mm Petri dish holder (Choose dish model below) - $507+

    NuncWillCoFalcon/In Vitro 35 mmCorning-MattekIn Vitro 29 mm

Gas bubblers for slice oxygenation

Add gas bubblers to any gravity system, without regulator - $115Add gas bubblers to any gravity system - with regulator - $340Oxygen8® bubbled AND pressurized 35 or 60 ml syringes - $1,199

Do you need our interface cable for connecting your valve controller to interface hardware like the DigiData or ITC interface?

4-BNC cable to Axon Digidata 1320 - 1322A - $708-BNC cable to Digidata 1440 & 1550, National Instruments, others - $125Cable to Heka EPC-10 / ITC-1600 - $65Cable to Heka EPC-10 USB - $65USB A-B cable for ValveLink8.2 - $9No Thanks

Do you need brackets for mounting your valve controller in a 19" rack?

Yes - $20No

If you chose the ValveBank® controller, would you like our EasyCode® software to help program it before experiments?

No thanksEasyCode for Macintosh Classic with USB-to-serial adapter - $274EasyCode for PC (Windows 3.1-95) - $195EasyCode for PC (Windows 98SE, 2000, XP, 7, 8, 10) - $249

Depending on component choices, AutoMate Scientific perfusion systems include:
Controller, valves, reservoirs, ringstand, 1/16" i.d tubing and four-, eight-, or sixteen-into-one micro-manifold with flow regulator. Non-pressurized PTFE inert systems include drippers and a reservoir bracket.

Perfusion Pencil®

4-channel Pencil (perfusion manifold) - $2508-channel Pencil - $25016-channel Pencil for fast, local drug application (one tip included) - $385

    Additional tips for Perfusion Pencil, SmartSquirt and ThermoClamp (quantity/$35 ea.):

    100µm i.d.

    250µm i.d.

    360µm i.d.

Sub-millisecond piezo solution switcher

Yes - $9300No, theta glass piezo switcher

Fast exchange, open Oocyte chambers

80 mm - $25090 mm - $250100 mm - $250108 mm - $250110 mm - $250120 mm - $250

Petri dish perfusion chamber insert

Nunc, default 35mm dish - $195Corning - $195Falcon - $195

Extra tubing
Minimum, 10ft. Price per foot.

1/16" i.d. Tygon

1/16" o.d. PTFE inert

1/32" i.d. Silicone pinch valve

Preferred domestic (US/Canada) shipping method

Preferred distributor (if any)

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Determines the number of liquids that can be controlled. 4-channel ValveLink systems actually come with a ValveLink8.2 controller (we don’t make a ValveLink4), so they can easily be expanded later by adding more valves. 16-channel systems come with two controllers connected electronically to act as one unit. We can daisy-chain a perfusion system with up to 80 channels. Please contact us.

ValveLinks are less expensive than AutoMate Scientific’s ValveBank controllers and include manual pushbuttons, digital/TTL, analog and USB inputs to control valves. They are designed for easy control by your data acquisition software. ValveBanks include manual control, digital inputs, plus they can be programmed through their keypad and LCD screen or EasyCode® software from a Macintosh or PC-Windows. ValveBanks run user valve sequence ‘programs’ without a computer, and include eight programmable digital outputs for control of external devices. Entire ValveBank programs can also be triggered by a single TTL pulse.

See a comparison of ValveLink and ValveBank controllers.

Pinch valves – 1/32″ i.d. silicon tube passes through, and is pinched closed by the valve. No liquid comes into contact with the valve. Easiest valves to clean and switch tubing; however, switching times are increased to 10-20ms. Less expensive.

PTFE-inert valves – Excellent chemical and corrosion resistance. Non-stick surface resists particle and chemical deposits. Open and close in approximately 8-10 ms. More expensive.

SmartSquirt® – Pneumatic valves use air pressure to push precious solutions from small cryo tube reservoirs located near the prep.

More about SmartSquirt

Priced per set of four valves. An extra grounding wire attached to all valves extending back to the controller. This item is required for CE conformity in Europe, and highly recommended for all electrophysiology.

It must be ordered when purchasing the valves – it cannot be added later.

For anyone who needs:

  • Faster Switching
  • Steady flow-rate
  • Small diameter pipette delivery
  • Microliter fine delivery
  • Microinjecting

Pressurization will help give more consistent flow rates with any perfusion system. It will also help overcome many problems associated with bubbles in the tubing, although this should really be accomplished by warming your solutions above room temperature (to outgas them) BEFORE your experiments. AutoMate Scientific’s pressure upgrade was primarily designed for use with the PerfusionPencil – our multi-barrel manifold tip. It is difficult to obtain liquid flow through our 250 µm tip with just gravity, and impossible through the 100 µm tip (see chart below). Several psi of air pressure results in ideal flow rates and very fast switching times through the PerfusionPencil.

  • Can be added to any new or existing perfusion rig – from AutoMate Scientific, any 3rd party manufacturer, even homemade!
  • Connect to house air, or compressor (30 to 100 psi).
  • Precision regulator delivers zero to 10 psi.
  • Available with four, eight or sixteen channels.
  • Syringe reservoirs may also be placed in a water bath.
  • Sturdy stainless steel construction.
  • Includes 5 micro bowl filter and gauge.
  • Elegant design allows individual air control and easy refilling.

Build Your Own - Superfusion

Our valve controllers use DB-9, DB-15, and DB-25 connectors respectively for their digital inputs (similar connectors to serial ports on computers). Some data acquisition hardware (i.e. DigiData, ITC-16, etc.) or computer interface hardware (i.e. National Instruments) use BNC connectors for their digital outputs. Others offer custom digital ports. AutoMate offers a variety of special cables pre-made to connect the valve controllers to digital outputs. Or, if you plan on controlling valves from your computer, you can make such a cable yourself.

Program your valve and TTL on-off sequences with a Macintosh or PC-Windows compatible computer using graphic ‘click-and-drag’ timebars. Save and load unlimited programs to disk, print out program listings, then download your programs into the ValveBank’s memory in one simple step.

More about EasyCode®

  • 4, 8 or 16 micro-bore tubes to 1 outflow
  • For single-cell and patch delivery
  • Rapid solution change
  • Very small dead volume
  • Vacuum cross-contamination prevention (use one tube to vacuum the tip volume clear between solution changes)
  • For mounting on any micromanipulator

Build Your Own, Perfusion Pencil

A Xenopus oocyte perfusion chamber for use in automated and unattended experiments. One of the major characteristics of this chamber is a small working volume (~20 µl), which allows for fast solution exchange.

More about Oocyte Chamber

Build Your Own - Oocyte

Perfuse cells right in your Petri Dish. Cells cultured in Petri dishes are a popular research tool used in numerous applications, including patch clamping and intracellular ion probe imaging. True perfusion (continuous inflow and outflow) of solutions can be difficult to configure. This forces scientists to plate cells on cover slips for placement into specially designed perfusion chambers. However, such transfer is a time consuming process which introduces the potential for contamination plus additional expense.

The PCP-1 chamber was designed by scientists after years of patch clamp research to overcome this problem. Perfuse cells right in your Petri dish with any perfusion system and an optional PTFE-Inert manifold (sold separately). Ideal for inverted microscopy using optically clear Petri dishes. Adjustable metal suction tube included. Dimensions: 35mm, outside diameter x 20mm tall.

More about Petri Chambers

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