Low-noise Digital Patch Clamp Amplifier System

Sutter dPatch® Low-noise Digital Patch Clamp Amplifier

dPatch® Low-Noise Amplifiers with Integrated Data Acquisition and Software

The dPatch® amplifier system was built around a simple idea: What if we built a clean-sheet design that used the latest technology to make the next generation of patch clamp amplifiers? We hired the best hardware and software designers available in the industry, the same engineers who created the leading amplifiers already in the market. We asked them to design the best amplifier system possible, using the very latest in digital architecture, and pair it with a contemporary, easy-to-use, yet powerful software platform.

The resulting design represents a complete rethinking of how to best reduce noise and preserve signal to get the cleanest recordings possible, at a bandwidth that far exceeds anything else on the market. The dPatch amplifier system’s digital architecture uses state-of-the-art methods in signal processing, such as field-programmable gate arrays (FPGAs) and Arm Core processors – technologies unavailable when the leading amplifiers in the market were designed well over 20 years ago. The processing power of this design FINALLY enables fully integrated dynamic clamp, as well as digital capacitance and resistance compensation. The included SutterPatch® Software facilitates data acquisition, management and analysis with an intuitive and easy to learn interface.

Available in either a single- or dual-headstage configuration, the dPatch amplifier system’s architecture makes swapping headstages, or adding a second one to a single-headstage unit, a plug-and-play operation. The two headstages are independently configurable for either voltage clamp or FastFollower™ current clamp.

5 Mhz Sampling Rate, Up To 22-Bit Resolution
One unique feature with dPatch is the headstage data sampling system. Each headstage is continually sampled at 5 MHz. Output filtering has thirteen settings between 100 Hz and 1 MHz. A resolution of 18 bits is achieved at 1 MHz. For lower filter settings, automatic downsampling increases resolution while optimizing data rates. At a bandwidth setting of 1 kHz, the dPatch system provides a signal resolution of better than 22 bits.

No Active Cooling Required
Active cooling causes numerous problems that actually create more “noise” in the long run. Active cooling in amplifier headstages use Peltier cells, which cool the electronics for slightly better performance, but generate considerable heat on the opposite side of the cell. The heat generated causes thermal drift which makes it almost impossible to stay patched while doing single-channel work. This is THE MOST COMMON source of what users perceive as “manipulator drift”. As a company that makes micromanipulators, we are highly sensitive to the performance of the system within a complete electrophysiology rig.

Active cooling can help get a slightly better noise specification on paper, but in the real world the disadvantages far outweigh the slight gain in specsmanship. One of the development goals of the dPatch headstage was achieving a comparable noise performance at room temperature, without the need for a cooled headstage. In the two resistive feedback modes, the dPatch amplifier is even quieter than any of the competitor systems. In addition, the limited life expectancy of Peltier elements causes reliability concerns that we found unacceptable.

Built-In Data Acquisition System Means No Third-Party Computer Interface
Using a multiplexer-free design, the dPatch provides 8 fully differential analog input channels, 4 analog output channels, and 16 digital outputs (TTL). All I/O channels are sampled continuously (200 kHz for analog inputs, 250 kHz for analog and digital outputs) and available through the user interface.

Sutterpatch Software
The dPatch amplifier system, in combination with SutterPatch software, has been engineered to automatically capture and store all amplifier settings, stimulus information and external experiment parameters, and associate them in time with the raw data traces. This includes all amplifier and acquisition settings, as well as timing and progress of the experiment. Fully integrated computer control of the amplifier stages means that the acquisition software is aware of the internal state of the amplifier and digitizer at all times and can track any changes that may occur. This is independent of whether a change is triggered automatically or initiated by the user.

Dynamic Clamp – New Feature
The patented digital architecture of the dPatch amplifier system provides an ideal platform for dynamic clamp. The dPatch is powered by a system-on-chip which provides parallel processing across a Field Programmable Gate Array (FPGA) and two high-speed ARM core processors. Several sophisticated dynamic clamp models are implemented within this architecture. In each model, the update of the applied current values occurs without communication between the dPatch and a computer. Depending upon the complexity of the model, update rates of up to 500 kHz can be achieved. (read more on the SutterPatch Software page)

Tracking Of Other External Data
In addition to status changes in connected hardware that are automatically tracked, the researcher can manually trigger tags to document events like stimulus application using instruments not connected to the amplifier. Information about environmental parameters and a more detailed specification of sample properties can be recorded and stored with the raw data. A total of over 650 metadata attributes are supported. Examples include: animal species, genotype, date/time when a cell sample was prepared, recording solutions, pipette resistance, hardware properties, and detailed information about stimuli applied.

Data Visualization And Analysis
SutterPatch software has been designed to simplify the navigation and analysis of complex datasets. The scope window supports multiple view modes in both two-dimensional and an innovative three-dimensional display. The 3D view is particularly useful during assay development. Built on top of the latest version of the proven Igor Pro platform, SutterPatch combines native Igor Pro functionality with a wealth of features that are tailored to electrophysiology applications. Both the newcomer and the experienced user of patch clamp programs will feel comfortable using SutterPatch software.

Application modules provide focused functionality for particular applications.

Currently available:

  • Event Detection Module: A deconvolution algorithm that excels at detecting miniature synaptic events even on a noisy background.
  • Action Potential Analysis Module: Phase plane plot, timing and waveform statistics.
  • Camera Module: An easy way to document the identity and condition of the recorded cell.

A Laboratory Workhorse
While the dPatch System is ready for cutting-edge research, its feature set makes it immediately valuable in any lab setting.

  • Three headstage feedback ranges for optimal whole-cell and single-channel recording
  • Automated compensation of electrode and whole-cell capacitance
  • Series resistance compensation
  • Simple cabling, quick and easy set-up
  • High dynamic range of digitizer means no need for variable gain stages
  • High speed of digitizer means no concern about sample rate

Common Applications

  • Single-channel recordings
  • Auditory research and other rapidly changing signals
  • Tissue slice recordings
  • Cultured cell experiments
  • Cell line studies from adherent or dispersed cells
  • Optogenetics
  • Nanopore and nanogap research

Features

  • Fully integrated single- or dual-headstage patch clamp amplifier and data acquisition system ensures quick and easy setup
  • Optimized for single-channel and whole-cell patch clamp recordings in tissue slices, adherent or dissociated cells
  • Full computer control provides automated compensation of electrode and whole cell capacitance
  • Voltage clamp, FastFollower™ current clamp and fully integrated dynamic clamp capability for complete characterization of cells’ electrical activity
  • Line frequency reduction in SutterPatch
  • Software lock-in amplifier in SutterPatch for high-resolution capacitance measurements
  • High bandwidth enables characterization of the fastest signals
  • Three headstage feedback ranges for single-channel and whole-cell patch clamp recordings
  • Comprehensive digital compensation circuitry provides the utmost precision and signal fidelity
  • Bundled SutterPatch® software provides versatile data management, intuitive navigation and streamlined data analysis

Accessories

Ground Point
The Ground Point GP-17 provides reliable, low resistance connections for a star ground configuration, the proven method to avoid ground loops in any electrophysiology setup. Accepts 9 banana plugs + 8 bare wires up to 10 gauge or banana plugs. The baseplate mounts directly on imperial or metric air table tops with the included ¼-20 and M6 screws. Made of solid, machined brass with plated banana/clamp connectors.

Sutter Ground Point GP-17

dPatch Expansion Panel

Sutter dPatch Expansion Panel
Quick Start Guide
Brochure
Manual

Technical Specifications

Dimensions
dPatch: 19″ x 11″ x 3.5″ | 48.2cm x 28cm x 9cm

dPatch Preamplifier:7.6″ x 3.5″ x 1.2″ | 19.5cm x 9cm x 3cm

dPatch Headstage: 3.7″ x 1.1″ x 0.66″ | 9.5cm x 2.9cm x 1.7cm

Weight
dPatch: 15 lbs | 6.8 kg

Electrical
110/240 Volts
50/60 Hertz power line

*Patent No. US 10,393,727 B2

System Requirements

Minimum Configuration:
• Windows 10 (64-bit), or MacOS: 10.11, El Capitan or later
• Processor: Dual-core i5
• Memory: 8 GB
• Solid-state drive (SSD): 500 GB or greater
• Display Resolution: 1024 x 768 (XGA)
• 1 available USB 3.0 SuperSpeed port (on the main board, not a PCIx card or similar)

Recommended Configuration for Bandwidths of >50 kHz:
• Windows 10 (64-bit), or MacOS: 10.11, El Capitan or later
• Processor: Dual-core i5
• Memory: 16 GB
• Solid-state drive (SSD): 500 GB or greater
• Display resolution 1920 x 1080 (Full HD)
• 1 available USB 3.0 SuperSpeed port (on the main board, not a PCIx card or similar)

All Sutter instrument amplifier systems include SUTTERPATCH® Data Acquisition Management System and Analysis Software.

Notes

USB 3.0 ports are compatible with USB 2.0 High Speed specifications. Slower USB 2.0 ‘full-speed’ ports, which are sometimes found on older Windows PCs or USB add-in cards, are not supported.

To check for High Speed USB 2.0 or USB 3.0 on a PC computer running Windows, look in the Control Panel > Device Manager > Universal Serial Bus controllers section for “Enhanced” host controllers. As this does not provide any mapping information to the computer’s physical ports, and there can be a mix of USB port versions, you should check individual USB ports for USB 2.0/3.0 High Speed operational performance. As a visual indicator, USB 3.0 ports are often color coded blue.

USB hubs are not supported. USB add-in cards, even if they formally meet High Speed or Super Speed specifications, are not recommended. They are often architecturally configured as USB hubs and may lead to intermittent transfer errors that are hard to troubleshoot.

Operating systems installed within virtualization software platforms such as VMware and Parallels are not supported.

CE Mark
Restriction of Hazardous Substances Directive 2002/95/EC