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PolyAn Functionalized Coverslips

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Bio Poly Microarray

Functionalized Coverslips

For high-resolution microscopic imaging or scanning applications PolyAn offers functionalized coverslips. All of PolyAn’s standard functional coatings can also be applied to coverslip glass.

All coverslips have the standard dimension of 25 x 60mm. Type #1.5 is used as a standard thickness. All coverslips are packed in boxes of 5. Other functionalities and formats are available upon request. PolyAn also offers functionalized substrates that are coated with gold or other noble metals as well as waveguide materials.

2D-functionalized Coverslips

Functionalized-Coverslips-Table1

For cost-sensitive applications PolyAn has developed a range of 2D (2-dimensional)-reactive coverslips that are manufactured from high quality glass with an ultra-flat surface and low inherent fluorescence. The glass is coated with a thin silane layer that will covalently bind most types of bio-molecules. The defect-free surface features uniform silane layers that provide a high covalent coupling efficiency together with a very low background.

3D-functionalized Coverslips

3D-Surfaces made by Molecular Surface Engineering

MSE Process

PolyAn’s 3D-functionalised microarray Slides are functionalized with a three-dimensional (3D)-surface chemistry comprised of a long-chain polymer containing a defined number of reactive groups. This polymer is covalently linked to the surface of the coverslip.

Our MSE-technology gently binds the functional layer onto the surface without damaging the base substrate. The morphology of the functional surface and thus the number of the reactive groups can be fine-tuned within a narrow range. This yields a number of advantages:

Low fluorescence background
Covalent binding of functional layer on the substrate without changing the initial autofluorescence.

Low unspecific binding
Combination of reactive functional groups with PolyAn antifouling matrix.

Topography
Tuneable surface
hydrophilicity / hydrophobicity (contact angle).

Uniform spot morphology
Narrow variation of surface properties and homogeneous distribution of reactive groups.

Optimal density and high accessibility of reactive groups
Morphology and thickness of functional layer tailored to the desired application.

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Specifications

Packaging
The coverslips are packaged in inert atmospheric boxes, with a capacity of 5 coverslips per box.

Storage
PolyAn’s coverslips are characterised by a long shelf-life when stored dry, at room temperature and protected from sunlight. All coverslips are packaged in boxes under Argon atmosphere to avoid contamination with particles. The Argon atmosphere also minimises degradation of the reactive surface through contact with air or humidity.