The most widely-used patch-clamp electrophysiology software is now even better.
pCLAMP 11 Software Suite
The Axon™ pCLAMP™ Software Suite is the most widely-used patch-clamp electrophysiology data acquisition and analysis program for control and recording of voltage-clamp, current-clamp, and patch-clamp experiments.
The software suite consists of:
- Axon Clampex 11 Software for data acquisition
- Axon AxoScope 11 Software for background recording
- Axon Clampfit 11 Software for data analysis
- Clampfit Advanced Analysis module (optional) for sophisticated and streamlined analysis
What’s New in pCLAMP 11
- Clampex 11 Software offers unparalleled ease of use for data acquisition.
- Enhanced Protocol Editor enables up to 50 Epochs in Episodic Stimulation (5x more Epochs than Clampfit 10), with the sweep duration increased up to 516 seconds at 10 kHz sampling. Now you can create more complex and flexible experimental protocols easily.
- The redesigned Membrane Test window displays up to eight channels simultaneously in one view. This parallel viewing allows easier control of cells and enables switching between cells much faster.
- Gap-free recording is a simple continuous “chart-recorder” recording mode useful for monitoring single channel events, minis, and other spontaneous activities. New in Clampex 11, users can now execute protocol-editor-style Epochs and program digital or analog outputs in Gap-free recording.
Clampex 11 Software—Flexible Data Acquisition
Clampex 11 is built on the previous version 10, which already has a powerful built-in feature set.
Enhancements include:
- Improved Protocol Editor—features up to 50 Epochs and 5X increase sweep duration
- Enhanced Gap-free mode—able to execute episodic-style epochs and program of digital and analog outputs
- Redesigned Membrane Test—enables viewing and individual controlling of multiple channels simultaneously in the same window
Clampfit 11 Software—Smart Data Analysis
Clampfit 11 Software offers a comprehensive palette of tools for data analysis.
Highlights include:
- Up to 25 cursor pairs allow more selection for regions of interest
- Graph plotting tools such as I-V plot and histogram
- Data conditioning such as filtering and baseline adjustment
- Fitting and statistical analysis
- Event detection including Template Search, Threshold search, and Single-channel search
Clampfit Advanced Analysis Module—Sophisticated Data Analysis
This module is a set of tools that expand the capabilities of Clampfit to combine powerful analyses with ease-of-use.
Highlights include:
- New Batch Data Analysis Tool—apply macros to analyze acquired data, thus saving time and reducing repetitive programming
- Improved Automated Event Detection—identify events based on user-defined parameters, which now includes populations spikes and paired pulses
- New Population Spike Analysis Tool—automatically calculates amplitude, area under the curve, coastline of spikes, etc.
- New Action Potential Analysis Tool—determines automatically the amplitude, APD90, rise & decay time, rise & decay slope, etc.
Requirements
Minimum System
- Windows 7 Pro (32-bit)**
- PC with 2 GHz processor
- 1.2 GB RAM
- 1440 x 900 display
- 1 USB 1 port (for security key dongle)
- 1 USB 2.0 port (for Digidata digitizers)
Recommended System
- Windows 10 Pro (32- or 64-bit)**
- PC with 2.0 GHz (or faster) Duo Processor
- 4 GB or higher
- 1920 x 1080 display or higher
- 3 USB 2.0 ports
**Professional, Enterprise, and Ultimate editions but not Home edition.
Supported Hardware
- Digidata 1550B 8-Channel Digitizer
- Digidata 1440A 16-Channel Digitizer
- MiniDigi 1B Two-Channel Digitizer
- Axoclamp 900A Microelectrode Amplifier
- Axopatch 200B Microelectrode Amplifier
- MultiClamp 700B Microelectrode Amplifier
- Most BNC-telegraphed amplifiers also supported
pCLAMP Videos & Demos
pCLAMP 11 tips, demonstrations, webinars, and more.
References – pClamp
- Mechanisms for the Potassium Sparing Effects of Furo-semide in Mice on High Potassium Diets University of Nebraska Medical Center (Dissertation), May 2018Bangchen Wang
- Novel alternative splicing of GABA receptor RDL exon 9 from Laodelphax striatellus modulates agonist potency Insect Science, April 2020Jie Jiang, Li‐Xin Huang, Feng Chen, Cheng‐Wang Sheng, Qiu‐Tang Huang, Zhao‐Jun Han, Chun‐Qing Zhao
- The mechanisms of action of water-soluble aminohexanoic and malonic adducts of fullerene C60 with hexamethonium on model lipid membranes, Biochimica et Biophysica Acta (BBA) - Biomembranes, Volume 1862, Issue 11, November 2020S.S. Efimova, D.A. Khaleneva, E.V. Litasova, L.B. Piotrovskiy, O.S. Ostroumova,
- Pharmacological evaluation of clinically relevant concentrations of (2R,6R)-hydroxynorketamine Neuropharmacology, Vol. 153, Pages 73-81, July 2019Christopher L. Shaffer, Jason K. Dutra, Wei Chou Tseng, Mark L. Weber, Luke J. Bogart, Katherine Hales, Jincheng Pang, Dmitri Volfson, Christopher W.am Ende, Michael E. Green, Derek L.Buhl
- Functional characterization of two spliced variants of fructose gustatory receptor in the diamondback moth, Plutella xylostella Pesticide Biochemistry and Physiology, December 2019Xiao-Long Liu, Si-Jie Sun, Wen Hou, Jin Zhang, Qi Yan, Shuang-Lin Dong
- Functional characterization of pheromone receptors in the moth Athetis dissimilis (Lepidoptera: Noctuidae) Pesticide Biochemistry and Physiology, April 2019Xiao-Long Liu, Si-Jie Sun, Sajjad Ali Khuhro, Mohammed Esmail Abdalla Elzaki, Qi Yan, Shuang-Lin Dong
- Fiprole insecticide resistance of Laodelphax striatellus: electrophysiological and molecular docking characterization of A2′N RDL GABA receptors Pest Management Science, Vol. 74, Issue 11, November 2018, Pages 2645-2651Cheng‐Wang Sheng, John E Casida, Kathleen A Durkin, Feng Chen, Zhao‐Jun Han, Chun‐Qing Zhao
- Dynamic modulation of activity in cerebellar nuclei neurons during pavlovian eyeblink conditioning in mice eLife 2017;6:e28132.Michiel M ten Brinke, Shane A Heiney, Xiaolu Wang, Martina Proietti-Onori, Henk-Jan Boele, Jacob Bakermans, Javier F Medina, Zhenyu Gao, Chris I De Zeeuw
- Differential Coding Strategies in Glutamatergic and GABAergic Neurons in the Medial Cerebellar Nucleus Journal of Neuroscience, January 2020Orçun Orkan Özcan, Xiaolu Wang, Francesca Binda, Kevin Dorgans, Chris I. De Zeeuw, Zhenyu Gao, Ad Aertsen, Arvind Kumar and Philippe Isope
- A Gustatory Receptor GR8 Tunes Specifically to D-Fructose in the Common Cutworm, Spodoptera litura Insects 2019, 10, 272; doi:10.3390/insects10090272, August 2019Xiao-Long Liu, Qi Yan, Yi-Lin Yang, Wen Hou , Chun-Li Miao, Ying-Chuan Peng, Shuang-Lin Dong
- 1,3-Thiazine, 1,2,3,4-Dithiadiazole, and Thiohydrazide Derivatives Affect Lipid Bilayer Properties and Ion-Permeable Pores Induced by Antifungals Front. Cell Dev. Biol. June 2020Anastasiia A. Zakharova, Svetlana S. Efimova, Valeriy N. Yuskovets, Igor P. Yakovlev, Zara M. Sarkisyan and Olga S. Ostroumova