Electrode-Puller for fabrication of intracellular Glass- Microelectrodes, Injection-Microelectrodes and Patch electrodes.
Fully automatic polishing of Patch electrodes after pulling and microprocessor controlled capable of:
- Pulling low resistance electrodes from thick-walled and thin-walled borosilicate glass up to 2 mm o.d. The electrodes are sturdy, reliable, and the instrument fabricates pipettes with high reproducibility.
- Pulling high-resistance electrodes up to 100 Mohms.
- Longitudinal distance of pull
- Force of pull, time controlled program
- Force of pull, distance controlled programm
- Time controlled or distance controlled heat application
- Power of heating, regulated by optical sensor
- Fully automatic fixation and release of the glass tubing
- Fully automatic polishing of the two tips
The DMZ-Universal-Electrode-Puller consists of an unique pulling mechanism which was developed at Institute of Physiology in Munich, Germany. It is nearly free of any friction and precise enough to produce pipettes with a tip diameter down to 0.2 µm. Although the device pulls on only one end of the capillary glass, it is able to make both pipettes equal by re-centering the glass tube after each elongation. With this construction it is possible to produce pipettes and electrodes for a great variety of different purposes. Additionally the device is designed to not only produce single pipettes, but to reproduce pipettes with the exact shape, taper and tip diameter immediately before use.
DMZ-Universal-Electrode-Puller in action
80 programs can be stored permanently, all variables can be set independently. One- or two-stage pulling programs, in which all variables can be set independently for each stage, with the option of repeating the first stage. The puller is fully automatic. There is no risk of breaking thin-walled glass capillaries during fixation because two micromotors fix the ends of the glass tube by clamping them with precisely defined force. Even with three-stage-pulling, the two tips are symmetrical because the position of the glass tube relative to the heat filament is readjusted after each stage of pulling.
There is no need for air-jet cooling because the duration of heat application is controlled by programmable approach and withdrawal of the (U-shaped) heating filament. The program can include sensing of stiffness of the tubing during heating which compensates for variations in the wall of the tubing. Free choice and absolute reproducibility of tip size (down to 0.1 micrometer), taper and electrode resistance (up to 100MOhms). Especially suitable for pulling patch electrodes of precisely defined tip diameter (usually 1-30 micrometer). Such electrodes can also be polished by the puller.
The heating filament is made of KANTHAL©, a special high-temperature alloy. Although KANTHAL© is extremely robust and durable, the extremely precise control of filament heat, a prerequisite for the puller’s exceptional pipette reproducibility, requires that the filament heating time constant be as low as possible, Since this limits the lifetime of the filament, it is important that the filament be replaced in good time so as to maintain the pipette reproducibility. Compared to other pullers, the range of electrode tip sizes and shapes is relatively independent of the precise shape and position of the heating filament. Therefore repeated readjustment of the heating filament is not necessary.
Because of the excellent reproducibility of the electrodes (even over months) the DMZ Universal puller is suitable for pulling electrodes immediately before use. Prefabrication of electrodes before the experiment is no longer required.
Whole cell pipette with a tip diameter of 5 µm
Patch-clamp pipette with a tip diameter of 2 µm
Minimum glass length: 75 mm
Minimum glass diameter: 1.0 mm
Maximum glass diameter: 2.0 mm
Width: 44 cm
Depth: 30 cm
Height: 25 cm
Weight: 29 kg
115/230 VAC: 4.0/2.0 A
(100 VAC also possible )
References – Zeitz DMZ puller
- Purification of Human and Mammalian Membrane Proteins Expressed in Xenopus laevis Frog Oocytes for Structural Studies Heterologous Expression of Membrane Proteins, pp 223-242 |03 August 2016Rajendra Boggavarapu, Stephan Hirschi, Daniel Harder, Marcel Meury, Zöhre Ucurum, Marc J. Bergeron, Dimitrios Fotiadis
- Long-Term Potentiation by Theta-Burst Stimulation Using Extracellular Field Potential Recordings in Acute Hippocampal Slice Cold Spring Harb Protoc 2016.doi:10.1101/pdb.prot091298Therese Abrahamsson, Txomin Lalanne, Alanna J. Watt and P. Jesper Sjöström
- Genetic and molecular studies of skeletal muscle channelopathies Durran Thesis, 2016Siobhan Durran