3D-Azide surface chemistry for bio-orthogonal binding

of oligonucleotides, peptides and small molecules containing cyclo-alkyne groups, e.g. DBCO (Dibenzocyclooctyne) for copper-free SPAAC – Strain Promoted Alkyne-Azide Cycloaddition or alkyne groups for copper-catalyzed CuAAC.

Key Features:

  • Simple coupling chemistry, highly specific bio-orthogonal reaction
  • Alternative to conventional Streptavidin-Biotin immobilization
  • Three-dimensional (3D)-surface chemistry comprised of a long-chain polymer containing a defined number of azide groups. The 3D-structure incorporates moieties that reduce unspecific binding
  • Polypropylene plates available for applications using organic solvents or aggressive media

Application example: Immobilization of oligonucleotides via copper-free click-chemistry

  1. Immobilization 5’-DBCO-oligonucleotide (20mer) with 3’-6-FAM label via SPAAC in buffer
  2. Hybridization with 5’-TAMRA anti-strand in bufferII

PolyAn equips conventional 96-well plates as well as solvent stable Polypropylene plates with 3D-Azide surfaces.

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Notes

Please do not hesitate to contact us if you would  like to functionalize a different format or substrate with our 3D-Azide surface.