PolyAn Microparticles Overview 

PolyAn offers transparent polymer beads with a very narrow particle size distribution for particle analysis using flow cytometry or other screening applications.

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PolyAn offers functionalized, transparent polymer beads with a very narrow particle size distribution for particle analysis using flow cytometry or other screening applications. All of our PolyAn beads are available with 3D-Carboxy, Streptavidin, Neutravidin and 3D-Alkyne. A custom modification with Peptides or Oligonucleotides is available upon request.

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PolyAn’s fluorescent microparticles are available in various sizes, emission spectra and fluorescence intensities. The fluorescent PMMA microparticles are suitable for use in flow cytometry, fluorescence microscopy, phagocytosis studies and cell labelling. They can be used in image based systems as well as in other screening applications. Typical applications include calibration of flow cytometers, calibration of fluorescence microscopes and multiplex bead assays.

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PolyAn offers a wide variety of functionalized, fluorescent particles for use in flow cytometry and image based systems as well as other imaging systems. PolyAn also offers different multiplex bead sets for flow cytometry and fluorescence microscopes. The fluorescence intensity of the beads can be tailored to specific applications and read-out systems.

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PolyAn’s hydrophobic beads can be applied to immobilize amphiphilic biomolecules like phospholipids, lipopolysaccharides, lipoproteins, or even membrane-based receptors after cell-lysis. The hydrophobic beads are characterized by a high contact angle with water, and are stable in organic solvents such as methanol, acetone and even chloroform (up to 50% v/v).

Hydrophobic Beads

PolyAn Plex bead kits provide a platform for the design of multiplexed suspension arrays that can be run on standard flow cytometers. PolyAn offers a 25-plex (peaks) set of beads that can be distinguished by both different fluorescence intensities of our PolyAn Red4 dye (Excitation: 590–680 nm/Emission: 660–780 nm) as well as three different sizes (3.5 μm, 5 μm and 9 μm).

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PolyAn’s Spectrum Calibration Beads are designed for calibration of flow cytometers and other fluorescence imaging systems. Each color encoded PMMA bead population (peak) contains a mixture of fluorophores that allows performance validation at all wavelengths.

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