Brain Slice Keeper 12 – twelve wells within same 500ml volume
Brain Slice Keeper 12 – twelve wells within same 500ml volume
$994.00
Description
Brain Slice Keeper 12 (BSK12)
The Brain Slice Keeper 12 (BSK12) has been designed to pre-incubate a large number of brain slices prior to transfer into recording chambers or for the incubation of preparations in experimental test media. This keeper consists of twelve closely fitting acrylic ‘C’ rings that fit into their own through-hole, located in a circular manifold. Removable netting is wedged between the ‘C’ ring and it’s hole.
In operation, the twelve rings located on a manifold with netting are totally immersed in an ACSF-filled purpose designed holding vessel into which a 95% O2, 5% CO2 gas mixture is supplied through a ceramic bubbler. The ceramic bubbler is located in a central tube chamber connected to the upper manifold. The bubbles rising from the base saturate the ACSF and provide constant circulation of medium to the slices which rest on the nylon nets.
Features:
- Slices maintained for many hours in incubating media
- Twelve separate rings allow separation of different types of preparations
(all in the same bath solution) - Slices supported on a quick-change nylon net
- Modular design simple to set up and maintain
Bubbles are restricted to rising in the central chamber and are prevented from being trapped under the slices, thereby ensuring a continuous circulation of medium. Slices remain viable for many hours in these conditions. The BSK12 together with the holding vessel can be easily placed into a water bath for regulating the incubating temperature as desired. A lid with a conical profile ensures that condensation drops do not fall directly above the slices.
When in place, the fluid level is maintained at least 3mm above the ’C’ rings. This ensures circulation of fluid over the top and downwards towards the slices. The typical fluid volume with BSK12 is 500ml but can be reduced by adding large glass spheres into the base.
Hold more brain slices separately than any other chamber
Schematic Diagram
Schematic arrangement of BSK12 and location in purpose designed trough with conical profiled lid.
Construction:
A central bubble chamber containing a ceramic air stone induces a constant flow of media over the multiple rings arranged in the circumference of the upper manifold. Each ring consists of a removable ‘C’ ring with netting inserted within a through-hole. Slices resting on the netting are bathed by the gentle downward circulating oxygenated solution which is then re-circulated through a space in the base. This direction of flow ensures slices maintain their position of the netting and no bubbles are trapped under the slices.
References – Brain Slice Keeper
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- Studies on CRMP2 SUMOylation-deficient transgenic mice identify sex-specific NaV1.7 regulation in the pathogenesis of chronic neuropathic pain Biorxiv, April 2020Aubin Moutal, Song Cai, Jie Yu, Harrison J. Stratton, Aude Chefdeville, Kimberly Gomez, Dongzhi Ran, Cynthia L. Madura, Lisa Boinon, Maira Soto, Yuan Zhou, Zhiming Shan, Lindsey A. Chew, Kathleen E. Rodgers, Rajesh Khanna
- Preparation of Rat Organotypic Hippocampal Slice Cultures Using the Membrane-Interface Method Patch Clamp Electrophysiology. Methods in Molecular Biology, vol 2188. Humana, New York, NY. October 2020Timothy W. Church, Matthew G. Gold
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- Preparation of Acute Brain Slices Using an Optimized N-Methyl-D-glucamine Protective Recovery Method Jove, Issue 132, e53825, Feb 2018Ting, J. T., Lee, B. R., Chong, P., Soler-Llavina, G., Cobbs, C., Koch, C., Zeng, H., Lein, E.
- Opening of KATP Channel Regulates Tonic Currents From Pyramidal Neurons in Rat Brain Canadian Journal of Neurological Sciences, Vol. 44, Issue 6 November 2017, pp. 718-725Zhongxia Li, Jiangping Wang, Huimin Yu and Kewen Jiang
- Neocortical Microdissection at Columnar and Laminar Resolution for Molecular Interrogation Current Protocols in Neuroscience, Vol. 86, Issue 1, January 2019Koen Kole, Tansu Celikel
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- h-Channels Contribute to Divergent Intrinsic Membrane Properties of Supragranular Pyramidal Neurons in Human versus Mouse Cerebral Cortex Neuron, Vol. 100, Issue 5, Pages 1194-1208.e5, December 2018Brian E. Kalmbach, Anatoly Buchin, Brian Long, Jennie Close, Anirban Nandi, Jeremy A.Miller, Trygve E. Bakken, Rebecca D.Hodge, Peter Chong, Rebecca de Frates, Kael Dai, Zoe Maltzer, Philip R. Nicovich, C. Dirk, Keene, Daniel L. Silbergeld, Ryder P. Gwinn, Charles Cobbs, Andrew L.Ko, Jonathan T.Ting
- Developmental Excitation of Corticothalamic Neurons by Nicotinic Acetylcholine Receptors J. Neurosci. 2008; 28(35): p. 8756-8764Sameera M. Kassam, Patrick M. Herman, Nathalie M. Goodfellow, Nyresa C. Alves, and Evelyn K. Lambe
- Betaine in the Brain: Characterization of Betaine Uptake, its Influence on Other Osmolytes and its Potential Role in Neuroprotection from Osmotic Neurochemical Research, Vol. 42, Issue 12, pp 3490–3503, December 2017Leena S. Knight, Quinn Piibe, Ian Lambie, Christopher Perkins, Paul H. Yancey
- ARCAgRP/NPY Neuron Activity Is Required for Acute Exercise-Induced Food Intake in Un-Trained Mice Front. Physiol., May 2020Wyatt Bunner, Taylor Landry, Brenton Thomas Laing, Peixin Li, Zhijian Rao, Yuan Yuan and Hu Huang
- Apamin Improves Prefrontal Nicotinic Impairment in Mouse Model of Alzheimer's Disease Cerebral Cortex, bhz107, June 2019É Proulx, S K Power, D K Oliver, D Sargin, J McLaurin, E K Lambe
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- AgRP/NPY Neuron Excitability Is Modulated by Metabotropic Glutamate Receptor 1 During Fasting Front Cell Neurosci. 2018; 12: 276.Brenton T. Laing, Peixin Li, Cameron A. Schmidt, Wyatt Bunner, Yuan Yuan, Taylor Landry, Amber Prete, Joseph M. McClung, and Hu Huang
- A robust ex vivo experimental platform for molecular-genetic dissection of adult human neocortical cell types and circuits Nature Scientific Reports, Vol. 8, Article number: 8407 (2018)Jonathan T. Ting, Brian Kalmbach, Peter Chong, Rebecca de Frates, C. Dirk Keene, Ryder P. Gwinn, Charles Cobbs, Andrew L. Ko, Jeffrey G. Ojemann, Richard G. Ellenbogen, Christof Koch & Ed Lein
Additional information
Weight | 2 lbs |
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