Brain Slice Keeper 6 – low 4ml volume x 6 wells
Brain Slice Keeper 6 – low 4ml volume x 6 wells
$1,808.00
Description
Brain Slice Keeper (BSK 6)
The Brain Slice Keeper (BSK 6) Brain Slice Keeper has been designed to pre-incubate six separate sets of brain slices in solution volumes of 4ml prior to transfer into recording chambers. This enables slices to be pre-loaded with experimental test solutions that are too expensive for large volume dilutions. It consists of a large acrylic disc into which is machined six completely independent wells.
Each well has a closely fitting acrylic ring between which is wedged a removable sheet of nylon netting. The design of each well is such that when bubbled by an attached needle, a stream of oxygen bubbles introduced from the side are made to circulate the solution from the top downwards on to the net whilst also saturating the solution with oxygen. A channel below the net re-circulates the solution and exerts a downward force on the slices holding them down on the net.
Features:
- Minimum 4ml per incubating well for expensive test solutions
- Modular design allows efficient cleaning between experiments
- Slices supported on a quick-change nylon net
- Simple to set up and maintain
In operation, each of the BSK6-6 wells is filled with ACSF until the ‘C’ ring insert is totally immersed at least 1mm above the rim. Each well is supplied with a separate feed of 95% O2, 5% CO2 mixture via a needle submerged at one end of the well. The feed of the gas mixture to each well is from six fine flow gas regulators, supplied with the slice keeper. These in turn are supplied from a 1-into-6 gas input manifold also supplied with the chamber. Before introducing slices the wells are over-supplied with oxygen to saturate the incubating media.
After a few minutes the oxygen flow rate is reduced and the BSK6-6 is ready to accept one or two slices per well which are placed on the net. After several uses the nylon net can be replaced by pulling the acrylic ‘C’ ring and wedging a new sheet in between.
Designed to pre-incubate six separate sets of brain slices
Schematic Diagram
Schematic arrangement of BSK6-6 with detail of a single well including a cross-section view.
Construction:
A blunted 18G hypodermic needle [A] is inserted into a plastic fitting [B] and screwed into the chamber at [C]. The blunted tip is bent at 90˚ and used to bubble the gas mixture which creates a flow of solution towards the slice located on the nylon netting, which is in turn held by a tightly fitting ‘C’ ring [E]. The flow path continues under an acrylic plug [D] returning back to the bubbling region to complete the cycle (green arrows in lower drawing) The acrylic plug is normally held in place by the ‘C’ ring but can be lifted out when cleaning the slice keeper. The nylon netting [F] is placed over the well [G] and the ‘C’ ring [E] pushed down over it and the excess is removed by cutting with a new scalpel blade or small scissors.
References – Brain Slice Keeper
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- The abnormal firing of Purkinje cells in the knockin mouse model of DYT1 dystonia Brain Research Bulletin, September 2020Yuning Liu, Hong Xing, Bradley J. Wilkes, Fumiaki Yokoi, Huanxin Chen, David E. Vaillancourt, Yuqing Li
- Studies on CRMP2 SUMOylation-deficient transgenic mice identify sex-specific NaV1.7 regulation in the pathogenesis of chronic neuropathic pain Biorxiv, April 2020Aubin Moutal, Song Cai, Jie Yu, Harrison J. Stratton, Aude Chefdeville, Kimberly Gomez, Dongzhi Ran, Cynthia L. Madura, Lisa Boinon, Maira Soto, Yuan Zhou, Zhiming Shan, Lindsey A. Chew, Kathleen E. Rodgers, Rajesh Khanna
- Preparation of Rat Organotypic Hippocampal Slice Cultures Using the Membrane-Interface Method Patch Clamp Electrophysiology. Methods in Molecular Biology, vol 2188. Humana, New York, NY. October 2020Timothy W. Church, Matthew G. Gold
- Preparing Viable Hippocampal Slices from Adult Mice for the Study of Sharp Wave-ripples Bio-protocol 10(19): e3771, October 2020Liu, L., Zhou, X. and Wu, J.
- Preparation of Acute Brain Slices Using an Optimized N-Methyl-D-glucamine Protective Recovery Method Jove, Issue 132, e53825, Feb 2018Ting, J. T., Lee, B. R., Chong, P., Soler-Llavina, G., Cobbs, C., Koch, C., Zeng, H., Lein, E.
- Opening of KATP Channel Regulates Tonic Currents From Pyramidal Neurons in Rat Brain Canadian Journal of Neurological Sciences, Vol. 44, Issue 6 November 2017, pp. 718-725Zhongxia Li, Jiangping Wang, Huimin Yu and Kewen Jiang
- Neocortical Microdissection at Columnar and Laminar Resolution for Molecular Interrogation Current Protocols in Neuroscience, Vol. 86, Issue 1, January 2019Koen Kole, Tansu Celikel
- Impaired Cholinergic Excitation of Prefrontal Attention Circuitry in the TgCRND8 Model of Alzheimer's Disease J. Neurosci. 2015; 35(37): p. 12779-12791Eliane Proulx, Paul Fraser, JoAnne McLaurin, and Evelyn K. Lambe
- h-Channels Contribute to Divergent Intrinsic Membrane Properties of Supragranular Pyramidal Neurons in Human versus Mouse Cerebral Cortex Neuron, Vol. 100, Issue 5, Pages 1194-1208.e5, December 2018Brian E. Kalmbach, Anatoly Buchin, Brian Long, Jennie Close, Anirban Nandi, Jeremy A.Miller, Trygve E. Bakken, Rebecca D.Hodge, Peter Chong, Rebecca de Frates, Kael Dai, Zoe Maltzer, Philip R. Nicovich, C. Dirk, Keene, Daniel L. Silbergeld, Ryder P. Gwinn, Charles Cobbs, Andrew L.Ko, Jonathan T.Ting
- Developmental Excitation of Corticothalamic Neurons by Nicotinic Acetylcholine Receptors J. Neurosci. 2008; 28(35): p. 8756-8764Sameera M. Kassam, Patrick M. Herman, Nathalie M. Goodfellow, Nyresa C. Alves, and Evelyn K. Lambe
- Betaine in the Brain: Characterization of Betaine Uptake, its Influence on Other Osmolytes and its Potential Role in Neuroprotection from Osmotic Neurochemical Research, Vol. 42, Issue 12, pp 3490–3503, December 2017Leena S. Knight, Quinn Piibe, Ian Lambie, Christopher Perkins, Paul H. Yancey
- ARCAgRP/NPY Neuron Activity Is Required for Acute Exercise-Induced Food Intake in Un-Trained Mice Front. Physiol., May 2020Wyatt Bunner, Taylor Landry, Brenton Thomas Laing, Peixin Li, Zhijian Rao, Yuan Yuan and Hu Huang
- Apamin Improves Prefrontal Nicotinic Impairment in Mouse Model of Alzheimer's Disease Cerebral Cortex, bhz107, June 2019É Proulx, S K Power, D K Oliver, D Sargin, J McLaurin, E K Lambe
- Alterations of the electrophysiological properties from cortical layer 5 pyramidal neurons in temporary rapamycin-treated rodent brain slices Neuroscience Letters, Vol. 612, 26 January 2016, Pages 80-86Keming Rena, Lijuan Chen, Guoxia Sheng, Jiangping Wang, Xiaoming Jin, Kewen Jiang
- AgRP/NPY Neuron Excitability Is Modulated by Metabotropic Glutamate Receptor 1 During Fasting Front Cell Neurosci. 2018; 12: 276.Brenton T. Laing, Peixin Li, Cameron A. Schmidt, Wyatt Bunner, Yuan Yuan, Taylor Landry, Amber Prete, Joseph M. McClung, and Hu Huang
- A robust ex vivo experimental platform for molecular-genetic dissection of adult human neocortical cell types and circuits Nature Scientific Reports, Vol. 8, Article number: 8407 (2018)Jonathan T. Ting, Brian Kalmbach, Peter Chong, Rebecca de Frates, C. Dirk Keene, Ryder P. Gwinn, Charles Cobbs, Andrew L. Ko, Jeffrey G. Ojemann, Richard G. Ellenbogen, Christof Koch & Ed Lein
Additional information
Weight | 2 lbs |
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