The Axopatch 200B provides ±250 mV of offset potential.
The convenient seal test may be used in voltage clamp mode (5 mV pulse) or in current clamp mode (50 pA (ß=1) or 500 pA (ß=0.1).
Dual Command Potentials
Two separate command potential inputs allow you to sum command input signals from two different sources. The back panel command is scaled to afford greater range (up to ±1V), and so is quite useful for electrochemical measurements.
The Holding Command of the Axopatch 200B has been enhanced over that of its predecessors with the addition of a X1 and X5 switch that allows you to choose either 0 – 200 mV or 0 -1 V ranges. An ON/OFF switch can disable this control when an external command from a computer is used to establish the holding potential.
Pipette Capacitance Compensation
In operation in both voltage- or current-clamp modes, controls the Magnitude and tau of two time constants, Fast and Slow.
Cell Capacitance Compensation
Compensate up to 100 pF (ß=1) or 1000 pF (ß=0.1) to allow recording from a large range of cell sizes.
Ten gain settings spanning a 1000-fold range may be selected to scale the output to the most desirable level.
The Axopatch 200B allows you to correct leak errors as they appear without adjusting your data-acquisition software settings.
A 4-pole Bessel filter with five settings from 1 kHz up to 100kHz allows you to set the bandpass appropriate to your application over a range double that previously available.
Reports values for current, voltage or headstage temperature while in any operational mode. Steady-state current is reported to monitor voltage-clamp output. To evaluate noise, current is displayed as an RMS value.
The Axopatch 200B facilitates patch rupturing by applying a single +1.3V pulse for a chosen duration.
Two HL-U holders are provided, of different barrel lengths, that accept pipettes of 1.0 mm to 1.7 mm OD.
A useful guide to the operation of the Axopatch 200B written by scientific consultants, with the assistance of Axon Instruments staff.