Microscope Stage Chamber: Submerged

The MS1 chamber has been designed for use with fixed-stage upright-type microscopes. The particular feature of this chamber is the profiled contour that allows the microscope objective to sweep across the preparation when changing magnification, without having to adjust the focusing. In this way the specimen and any pre-positioned microelectrodes remain par-focal. This is particularly useful for confocal microscopy where it is the intention to combine electrophysiology and precise localization of structures (Emptage, Bliss & Fine, 1999). The design also allows for movement of objectives that swing from front to back (see photo).

Perfusion solution enters from one side (right) via a stainless steel tube and exits through a well (left) in which a movable “L” shaped tube attached to a pump/suction line adjusts the fluid level. The profile slopes in towards the central coverslip base so that the perfusion solution forms a pool around the preparation whilst being refreshed by the flow created by the inlet and exit ports. Solutions can be pre-warmed before entering the chamber with our Mini Heating Module MH01 or ThermoClamp inline heater.


  • Size: 100mm diameter X 10mm thickness
  • Material: Clear acrylic
  • Coverslip base: 22mm X 150um thickness, borosilicate glass. Attached to base of chamber with silicone rubber sealant.
  • Inlet tube: Stainless steel tube, 1.81mm O.D. pushed into 10X32 UNF fitting.
  • Exit tube: As for inlet tube
  • Dead space: Min. capacity of pool formed above coverslip and channels leading to inlet and exit ports is 1ml.
Microscope Stage

References – Microscope Stage Chambers